8 thoughts on “C84 – Kooiman

    1. While we didn’t perform any statistical analysis, the slight decrease that was seen was very minor and thus wasn’t likely to have a p value below 0.05. Successive experiments need to be done to be able to confirm this decrease. However, as stated in my presentation, the qRT-PCR results allowed us to have more confidence in concluding that our Upec gene was down regulated under damage conditions.

    1. A western blow analysis is used to view protein concentrations under different conditions. This could be used in future experiments to see how damage results in protein production. Since our gene was decreased in expression under damage, it would be likely that there would be less protein production and thus a dimmer band on a western blot analysis. Overall, this would give us more information about the function of our gel under many conditions.

  1. Where were the T. Thermophila used in these experiments isolated from, and why did you use this organism specifically to test the Upec gene?

    1. During out experiments, we were provided the gene within T. Thermophila by Dr. Moore. As stated in the presentation, T. Thermophila was used at the model organism due to the prominence in double stranded breaks during conjugation that are then repaired. This just shows that T. Thermophila have highly developed DNA break repair machinery that was ideal for our research.

    1. I am a little confused by your questions because, as in my presentation, the central hypothesis questioned the expression of Upec gene under different conditions. The expected outcome, again as stated, was that due to the gene expression profiles there would be an increase in gene expression under damaging conditions. The use of T. Thermophila was becuase of the prominence in double stranded breaks during conjugation that are then repaired. This just shows that T. Thermophila have highly developed DNA break repair machinery that was ideal for our research.

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