4 thoughts on “C87 – Molison

  1. Given your results, what makes you suspect that Rfc1 is a protein associated with repair rather than another protein associated with cell damage or apoptosis?

    1. Hi Jordan, thanks for the question! As I mentioned in my presentation, we hypothesized that Rfc1 was involved in repair due to its expression patterns during conjugation. This process specifically involves DNA repair (and not something else such as apoptosis). We used this observation to make the prediction that Rfc1 was involved in repair (since its gene expression increased during this time) as opposed to another process. Additionally, Rfc1 is a homolog to subunit 1 (the large subunit) of replication factor C, which is a protein involved in replication/repair in other organisms, such as Homo sapiens. This connection also helped us make this initial hypothesis.

  2. Based on the variation shown in your data you collected (i.e. speaking specifically on the different result images shown), you said that Rfc1 most likely has other roles in conjugation outside of DNA repair. What other roles were you talking about, specifically?

    1. Great question Aarushi! So the full answer is – we don’t entirely know! One of the reasons we studied Rfc1 is to add possible insight into answering this exact location. Lots of other processes happen during conjugation (as this is sexual mating). For instance, there is a ton of cell division that occurs after pairing, as well as elongation of the nucleus (which enables homologous pairing). Rfc1 could very much be involved in one of these processes (or something else!) instead of repair.

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