20 thoughts on “D100 – Shilo

  1. How does Clofazimine’s mechanism of action apply to cancer cells? How does a growth suppressor target cancer?

    1. If we are able to suppressor the growth of cancer cells with the addiction of radiotherapy, there is a higher chance of treating the cancer to the point of remission, since remission is considered to be the redetection of the size of a cancerous tumor.

  2. Hi Ania! Great job on your poster presentation! It was very clear you understood your experiment well and you did a great job of conveying that. What was one of the most challenging things you came across when you were conducting your experiments?

    1. Thank you! I think one of the most challenging things for me is to understand how to complete the dilutions and how to make sure the experiment was conducted in the most accurate manner possible.

  3. I’ve heard a lot about how mice are also used as test subjects for medical research, so I was just wondering if you happen to know if there is an advantage for using drosophila melanogasters over mice or vice versa? You did a really good job, by the way!

  4. I’ve heard a lot about using mice in medical research, and this is the first time I’ve heard of using drosophila melanogaster as a test subject, so I was just wondering if you happened to know if there is an advantage, specifically with studying cancer therapies, with using one over the other?

    1. I think the best advantage is the fact that we are not conducting these experiments on in vivo organisms. It is extremely important to make sure that before moving a compound into an vivo organism research that we understand how it can effect healthy DNA. By using drosophila melanogaster, we were able to test an organism which has 70% similar DNA to humans, so if Clofazimine was used on in vivo organism we want to make sure it is safe to test.

  5. at the start you said that it is used as a growth supressor by changing the membrane’s asymmetry. that doesnt totally make sense to me. I would think that to be a growth supressor it would mess with a cell’s mitosis so that it cannto keep growing. could you explain a little more on how it changes the membrane’s asymmetry?

    1. Thank you for the comment! What I meant as a Clofazimine as a suppressor is that it reduces the sizes of the cell which means . Meaning, if the cell contains a mutation in a tumor suppressor then cell division would not be contained even if a toxic mutation was present. Clofazimine offers the opportunity to block the uncontrolled cell division which would reduce the likely hood of cancerous cells spreading.

  6. Hey good job on your presentation! I am wondering if you know anymore about clofazimine’s mechanism of action in its use as a treatment for leprosy??

    1. I unfortunately do not. I would love to know more though and how the cells of leprosy relate to cancerous cells and if there is a possible treatment for both.

  7. Great presentation! Why would you expect to see a different result when targeting a different gene in the same organism with the same compound?

    1. Thank you for the question! I think if we were able to test on genes which are specifically known to cause cancerous cells which have mutations in them, we would receive a more accurate result on our dilutions of the clofazimine.

    2. Thank you for your question! I think we would see a different result because we are testing on specific mutated genes which are known to cause cancer. For example, the BRCA1 gene is known to cause breast cancer if there is a mutation in it. If we were able to test Clofazimine on that gene with the mutation, we would receive more accurate results on if Clofazimine produces a hit.

  8. Great presentation! Why would you expect to observe a different result when targeting a different gene in the same organism and using the same compound?

    1. Thank you for your question! I think we would see a different result because we are testing on specific mutated genes which are known to cause cancer. For example, the BRCA1 gene is known to cause breast cancer if there is a mutation in it. If we were able to test Clofazimine on that gene with the mutation, we would receive more accurate results on if Clofazimine produces a hit.

  9. Do you know how the 70% similarity between the genes of Drosophila melanogaster and human genes was determined?

  10. I’m interesting in your idea of using cultures, have you done any research on how effective that is?

    1. My lab group and I have not done any research specifically but I think it would be worth to try since we would get more accurate results on how Clofazimine could effect specifically cancerous cells.

    2. We have not done any specific research but I think using cancer cell cultures would give us a more accurate result on how Clofazimine effects genes which have mutations that cause cancer.

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