8 thoughts on “D109 – Huso

  1. Even though the mushroom you tested did end up working as a potential anti-biotic, did you do any research about the other effects that specific mushroom might have on the human body?

    1. Due to my group not receiving our active compounds until very late in the semester we were very limited in our testing and further research of the compounds we received. However, since our testing indicated it was a drug of interest. The next step would be further testing which would lead to testing in mice. Since mice have very similar attributes to humans. After approval for further testing in humans, the results from the mice can then be effectively transferred to humans which would then lead to discovering a greater amount of the effects it potentially has on the human body.

  2. How effective is this antibiotic in the human body?
    Is there any possibility resistance occurs after prolonged use of this antibiotic from the mushroom?

    1. Yes, so there is always the chance of resistance which is due to many factors. Such as overprescribing and bacteria developing resistance mechanism from their DNA (genes change or get drug-resistant genes from other bacteria) No matter what, the longer and more often antibiotics are used, the less effective they are against bacteria. Due to receiving our Pleuromutilin extract very late, we were not able to continue with more tests. The next step would be testing in mice which would then lead to testing in humans. However, our testing exhibits our compound as a very promising antibiotic.

  3. What starting concentration of reishi mushroom would you propose be used in a mouse population study?

    1. Hi! we tested a stock solution of 1mg/mL of Pleuromutilin in our experiment. Due to receiving our active compounds late in the semester. We were not able to conduct a dose response experiment for the compound we determined as a HIT. The dose response experiment is diluting the stock solutions further to see how this effects the growth of the Salmonella. We would suggest testing this compound at different concentrations to determine the most effective concentration which can then be transferred over for use in testing in mice.

  4. If the mushroom has been used as an antibiotic for centuries, why has it not been studied further in modern times? Is there any reason other antibiotics were studied/used more readily?

    1. Hi! The Reishi mushroom has been used pharmaceutically in Eastern cultures which tends to use whole food formulas as treatments and do not undergo the vigorous testing process for approval like in the US. The rise of naturopathic medicine influenced by Eastern medicine has become more popular in Western medicine and have began testing natural therapies but is far from becoming a staple form of treatment. The reason why other antibiotics are used more readily is due to the lack of awareness of antibiotic resistance, over prescribing and lack of incentive to create new drugs and instead only changing already existing antibiotics.

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