the most effective dose is typically the highest dose that kills all of the bacteria so 0.4% is where it works as well as ampicillin, however the problem with killing ALL the bacteria is that it could kill the living cells. What the dilution series does is help pinpoint where we can start creating the dose response curve where there’s a change in effectiveness with a change in concentration. From the graph given it would be good to look into the concentration between 0.4% and 0.0004% where it kills bacteria as well as ampicillin at 0.4% and works as well at killing bacteria as water at 0.0004% after creating a response curve another test would need to be done on cells to figure out the level of cell toxicity. comparing the effective dose to the cell toxicity is where the compound could possibly work as an antibiotic.
Often times you want a drug that has low cell toxicity and broad window of effectiveness where you won’t die from an overdose or if you take too little it doesn’t do anything.
Bacteriostatic means that it prevents bacteria from reproducing and bactericidal means it kills the bacteria directly. Even though an antibiotic is bacteriostatic doesn’t mean it’s less effective than an antibiotic that kills bacteria straight up because preventing the bacteria from reproducing can provide valuable time for the body’s immune system to get rid of the bug by itself.
Based on your dilutions what was the most effective dose?
the most effective dose is typically the highest dose that kills all of the bacteria so 0.4% is where it works as well as ampicillin, however the problem with killing ALL the bacteria is that it could kill the living cells. What the dilution series does is help pinpoint where we can start creating the dose response curve where there’s a change in effectiveness with a change in concentration. From the graph given it would be good to look into the concentration between 0.4% and 0.0004% where it kills bacteria as well as ampicillin at 0.4% and works as well at killing bacteria as water at 0.0004% after creating a response curve another test would need to be done on cells to figure out the level of cell toxicity. comparing the effective dose to the cell toxicity is where the compound could possibly work as an antibiotic.
Often times you want a drug that has low cell toxicity and broad window of effectiveness where you won’t die from an overdose or if you take too little it doesn’t do anything.
What is the difference between bacteria static and bacteria styli?
Bacteriostatic means that it prevents bacteria from reproducing and bactericidal means it kills the bacteria directly. Even though an antibiotic is bacteriostatic doesn’t mean it’s less effective than an antibiotic that kills bacteria straight up because preventing the bacteria from reproducing can provide valuable time for the body’s immune system to get rid of the bug by itself.