Mice are what is being tested on in Corrie Detweiler’s lab. This is where the antibiotics that look like they might be able to be further developed are tested.
Thank you! I concluded that it was most likely that the bacteria was cultured incorrectly so the results on those days showed all low absorbance values (less bacteria). Instead of the results indicating that every dose and control killed the bacteria, it is likely that there was no bacteria in the wells to begin with.
Mice are what is being tested on in Corrie Detweiler’s lab. This is where the antibiotics that look like they might be able to be further developed are tested. In other labs, I have also heard of fish and other rodents being used similarly. However, this would be in the far future as this compound does need quite a lot more testing before that happens.
Thank you! Though we did have some unexpected data, those were likely due to experimental error and not anything related to our compound. From the results, we did have a dose that killed the bacteria, so it would be advantageous to continue testing to see if this could be an antibiotic that can be used in humans.
It has been considered yes. As what written on the poster, but not presented due to time constraints, it seems that it would be likely to be used in humans. The dose we proposed that would be the lowest to kill the bacteria is about 1000x lower than the dose currently used for chemotherapies.
Why would mice be your first choice to test on in the future?
Mice are what is being tested on in Corrie Detweiler’s lab. This is where the antibiotics that look like they might be able to be further developed are tested.
What do you think could have gone wrong? Great presentation by the way.
Thank you! I concluded that it was most likely that the bacteria was cultured incorrectly so the results on those days showed all low absorbance values (less bacteria). Instead of the results indicating that every dose and control killed the bacteria, it is likely that there was no bacteria in the wells to begin with.
Is there a specific dosage of azacicytidine that you would suggest to be tested and experimented on in the future?
I would suggest testing the dosages between 1uM and 0.5 uM to narrow down the concentrations to find the lowest effective dose.
Are there any other organisms you could test on in the future other than mice? Also, why use mice at first?
Mice are what is being tested on in Corrie Detweiler’s lab. This is where the antibiotics that look like they might be able to be further developed are tested. In other labs, I have also heard of fish and other rodents being used similarly. However, this would be in the far future as this compound does need quite a lot more testing before that happens.
Great job on your presentation! Despite your results, why do you think it’s worth it to continue investigating Azacytidine-5?
Thank you! Though we did have some unexpected data, those were likely due to experimental error and not anything related to our compound. From the results, we did have a dose that killed the bacteria, so it would be advantageous to continue testing to see if this could be an antibiotic that can be used in humans.
Have you considered the safety of this compound in human models?
It has been considered yes. As what written on the poster, but not presented due to time constraints, it seems that it would be likely to be used in humans. The dose we proposed that would be the lowest to kill the bacteria is about 1000x lower than the dose currently used for chemotherapies.