For starters we could use DMSO. It may not mimic the inside of a macrophage like our m9 media but it would serve as a buffer chemical and show us whether or not the m9 media had an inhibitory affect on our sulforaphane.
A very insightful presentation. How does Sulforaphane and compounds like it interact with the bacterial efflux pump? I noticed you mentioned that in the future research section.
Sulforaphane has been shown to regulate a specific protein associated with the formation of bacterial efflux pumps. This may be the reason for many of its observed antimicrobial and anticarcinogenic properties.
Any predictions I can make on the effect sulforaphane has on efflux pumps is based on other scientists’ research. Sulforaphane has been shown to regulate a specific protein associated with the formation of bacterial efflux pumps. Although our tests showed the compound sulforaphane as not having antibiotic properties, it may still have the ability to inhibit efflux pumps ability to alter the properties of human cells in certain gram negative bacteria.
What about sulforaphane being used as a supplement that promotes a healthy gut microbiome suggests that it could be used as an antibiotic? Is the idea that it would kill only some bacteria, or perhaps that it would help some bacteria outcompete others?
We didn’t add this thought to our future directions, but I personally considered the idea of a therapeutic method of fighting off “bad” bacteria by offering an environment supporting the growth of specific “good” bacteria. Further research would be required. Theoretically, this would be less effective than an antibiotic.
What about sulforaphane being an effective supplement for promoting a healthy gut microbiome suggests that it could be an effective antibiotic? Is the idea that it would only kill some bacteria, or something else?
Great question! This is relatively universal and a dosage that everyone in the discovery lab was recommended in starting with. It is also 1ug/mL instead of 1mg/mL. It seems the version of the poster used in my final presentation was not actually our final version of the poster. That was simply an error.
Great question! This is a universal max dose amount for the human body. It was recommended as a starting dosage for all of the discovery lab students. It is also 1 ug/mL. It seems I used an outdated version of our final poster in my presentation.
I think the M9 Media is great to use, what other media could you use to mimic that of a cell that would be useful in studying antibiotics?
For starters we could use DMSO. It may not mimic the inside of a macrophage like our m9 media but it would serve as a buffer chemical and show us whether or not the m9 media had an inhibitory affect on our sulforaphane.
A very insightful presentation. How does Sulforaphane and compounds like it interact with the bacterial efflux pump? I noticed you mentioned that in the future research section.
Sulforaphane has been shown to regulate a specific protein associated with the formation of bacterial efflux pumps. This may be the reason for many of its observed antimicrobial and anticarcinogenic properties.
What do you predict would happen to the function of the bacterium’s efflux pumps if you tested sulforaphane using other gram-negative bacteria?
Any predictions I can make on the effect sulforaphane has on efflux pumps is based on other scientists’ research. Sulforaphane has been shown to regulate a specific protein associated with the formation of bacterial efflux pumps. Although our tests showed the compound sulforaphane as not having antibiotic properties, it may still have the ability to inhibit efflux pumps ability to alter the properties of human cells in certain gram negative bacteria.
What about sulforaphane being used as a supplement that promotes a healthy gut microbiome suggests that it could be used as an antibiotic? Is the idea that it would kill only some bacteria, or perhaps that it would help some bacteria outcompete others?
We didn’t add this thought to our future directions, but I personally considered the idea of a therapeutic method of fighting off “bad” bacteria by offering an environment supporting the growth of specific “good” bacteria. Further research would be required. Theoretically, this would be less effective than an antibiotic.
What about sulforaphane being an effective supplement for promoting a healthy gut microbiome suggests that it could be an effective antibiotic? Is the idea that it would only kill some bacteria, or something else?
1mg/ml you said is the max dose used for the max dose test. Is that a universal constant, or was its something specific for you?
Great question! This is relatively universal and a dosage that everyone in the discovery lab was recommended in starting with. It is also 1ug/mL instead of 1mg/mL. It seems the version of the poster used in my final presentation was not actually our final version of the poster. That was simply an error.
Great question! This is a universal max dose amount for the human body. It was recommended as a starting dosage for all of the discovery lab students. It is also 1 ug/mL. It seems I used an outdated version of our final poster in my presentation.