In order for this to be an effective treatment, hypothetically, what dosage would you recommend giving to a patient, and what experiments would you do in the future to test this before trial on humans?
I would recommend a dosage of around 1.0 uM because this is a more effective and safe concentration (since it’s lower) than 3.0 uM, while having very similar results as ampicillin. I would first test the efficacy at even lower dosages to find the inverted U-curve and test other gram-negative bacteria with compounds similar to alexidine dihydrochloride to find its mechanism. Then, I’d proceed to animal testing before human trials.
I hope the Dottweiler Lab continues to experiment with various dosages of alexidine dihydrochloride before testing similar compounds and finding specific components of the drug that cause antibiotic effectiveness. I also hope the lab tests on if the compound is bacteriostatic or bactericidal.
Many antibiotics are used as chemotherapeutic agents because the mechanism used to kill the bacteria can also be used to kill our own tumors, which have an overwhelming amount of cells. Because of the overwhelming amount of bacteria that is targeted, we are hoping alexidine dihydrochloride can target these bacteria in the same way. More testing needs to be done on making sure that the compound targets bacteria more than just human cells in the body, however.
No, this is incredibly rare and is called a U-shaped dose response curve. It is shown to happen in certain compounds, namely cannabidiol (CBD) and veratrine (a drug that was actually used to treat Salmonella). Therefore, it’s definitely worth looking at more to see why alexidine dihydrochloride does this.
In order for this to be an effective treatment, hypothetically, what dosage would you recommend giving to a patient, and what experiments would you do in the future to test this before trial on humans?
I would recommend a dosage of around 1.0 uM because this is a more effective and safe concentration (since it’s lower) than 3.0 uM, while having very similar results as ampicillin. I would first test the efficacy at even lower dosages to find the inverted U-curve and test other gram-negative bacteria with compounds similar to alexidine dihydrochloride to find its mechanism. Then, I’d proceed to animal testing before human trials.
In the future, what do you hope to be done with this research?
I hope the Dottweiler Lab continues to experiment with various dosages of alexidine dihydrochloride before testing similar compounds and finding specific components of the drug that cause antibiotic effectiveness. I also hope the lab tests on if the compound is bacteriostatic or bactericidal.
If alexidine dihydrochloride is used in chemotherapy which is really harmful to the body, would it be harmful to use as a treatment for salmonela?
Many antibiotics are used as chemotherapeutic agents because the mechanism used to kill the bacteria can also be used to kill our own tumors, which have an overwhelming amount of cells. Because of the overwhelming amount of bacteria that is targeted, we are hoping alexidine dihydrochloride can target these bacteria in the same way. More testing needs to be done on making sure that the compound targets bacteria more than just human cells in the body, however.
Is this a common trait in drugs where decreasing the concentration causes a decrease in the bacteria?
No, this is incredibly rare and is called a U-shaped dose response curve. It is shown to happen in certain compounds, namely cannabidiol (CBD) and veratrine (a drug that was actually used to treat Salmonella). Therefore, it’s definitely worth looking at more to see why alexidine dihydrochloride does this.