Although I am not completely sure why this would be the case, one possible explanation could be that the dosage curve of the compound alexidine dihydrochloride is a U-shaped curve. This means that with further testing at even lower concentrations, the value on the y-axis (absorbance in the well) of the curve will increase again. This indicates that the trend in absorbance presented in the presentation could only be representative of a small part of this U-shaped curve where lower dosages are more effective than higher dosages. Thank you for your great question!
Now that you see bacterial death using alexidine, what type of experiment do you think would be most effective to perform next, in terms of continuing the drug development process?
The very next experiment I would suggest for this compound would be another drug screen test. This would be the same test that we have performed and presented here, however it should start with even lower concentrations of alexidine dihydrochloride. This would be most helpful in the drug development process because then we would be able to determine the smallest dosage that is still effective as a possible antibiotic, and also confirm (or deny) the possibility of the dosage curve being a U-shaped curve. Great question! Thank you for viewing the presentation!
Why do you think that the smaller doses were more effective than the larger doses?
Although I am not completely sure why this would be the case, one possible explanation could be that the dosage curve of the compound alexidine dihydrochloride is a U-shaped curve. This means that with further testing at even lower concentrations, the value on the y-axis (absorbance in the well) of the curve will increase again. This indicates that the trend in absorbance presented in the presentation could only be representative of a small part of this U-shaped curve where lower dosages are more effective than higher dosages. Thank you for your great question!
Now that you see bacterial death using alexidine, what type of experiment do you think would be most effective to perform next, in terms of continuing the drug development process?
The very next experiment I would suggest for this compound would be another drug screen test. This would be the same test that we have performed and presented here, however it should start with even lower concentrations of alexidine dihydrochloride. This would be most helpful in the drug development process because then we would be able to determine the smallest dosage that is still effective as a possible antibiotic, and also confirm (or deny) the possibility of the dosage curve being a U-shaped curve. Great question! Thank you for viewing the presentation!
It looks like you have really promising results… Is there anything you would try differently other than another drug screen with lower concentrations?