The next step in continuing this experiment would likely be to explore different experimental conditions. We could either test different chalcone derivatives that do not have the same solubility problems and show more potential, or we could try testing compounds in a different environment from the aqueous M9 media altogether.
Hi Ryan! Interesting results, if you were to do this lab over again, what considerations would take into selecting a new compound to test? (solubility, structure..etc)
One of the biggest considerations we would take into account is like you said, the solubility of the compounds. This was not something we initially expected to have to deal with, as it is soluble in DMSO, however we did not account for the fact that DMSO itself kills the bacteria. We also found research showing that more electronegative derivatives showed better effects so that may be a area we would look into.
If you had more resources and time within the lab, would you choose a different derivative of chlorine to use in this experiment, and what would be the best route of finding the best possible derivative to use?
We likely would choose a different chalcone derivative, and would probably choose one by conducting further research done in the past. We would pay special attention to the solubility of the compounds discussed in past research, as well as the solvents/concentrations used in order to try and best replicate the antibacterial results seen by others. We could also work to test a larger number of compounds just to determine whether or not an antibacterial effect can be seen.
You mentioned using Chalcones for your antibiotic research, but why was chalcone used in the first place? Does it have structural properties that are similar to antibiotics used today?
We mostly chose to explore chalcones due to previous research that showed antibacterial effects from them. We looked through several research articles that discussed the antibacterial properties of chalcones, and many of them discussed how they’re able to target bacterial systems that prevent the compound from being pumped out. They don’t exactly have many similar structural properties as known antibiotics, however, this is not entirely bad as the idea is that bacteria will not have seen the compound and won’t already exhibit resistance.
If you had more time in the lab or more resources, what would your next step be continuing with this experiment?
The next step in continuing this experiment would likely be to explore different experimental conditions. We could either test different chalcone derivatives that do not have the same solubility problems and show more potential, or we could try testing compounds in a different environment from the aqueous M9 media altogether.
Hi Ryan! Interesting results, if you were to do this lab over again, what considerations would take into selecting a new compound to test? (solubility, structure..etc)
One of the biggest considerations we would take into account is like you said, the solubility of the compounds. This was not something we initially expected to have to deal with, as it is soluble in DMSO, however we did not account for the fact that DMSO itself kills the bacteria. We also found research showing that more electronegative derivatives showed better effects so that may be a area we would look into.
If you had more resources and time within the lab, would you choose a different derivative of chlorine to use in this experiment, and what would be the best route of finding the best possible derivative to use?
We likely would choose a different chalcone derivative, and would probably choose one by conducting further research done in the past. We would pay special attention to the solubility of the compounds discussed in past research, as well as the solvents/concentrations used in order to try and best replicate the antibacterial results seen by others. We could also work to test a larger number of compounds just to determine whether or not an antibacterial effect can be seen.
You mentioned using Chalcones for your antibiotic research, but why was chalcone used in the first place? Does it have structural properties that are similar to antibiotics used today?
We mostly chose to explore chalcones due to previous research that showed antibacterial effects from them. We looked through several research articles that discussed the antibacterial properties of chalcones, and many of them discussed how they’re able to target bacterial systems that prevent the compound from being pumped out. They don’t exactly have many similar structural properties as known antibiotics, however, this is not entirely bad as the idea is that bacteria will not have seen the compound and won’t already exhibit resistance.
Awesome presentation!! You did amazing.