The most interesting part was how we tested the week old data on accident but had the best results with it! This was a week we were unable to go in the lab and the professor had tested this just for fun not really expecting anything and we got the best information from it.
What properties of the ink do you think makes this ink extract insoluble? What ideas do you have for a better solvent (ie amphipathic molecules / polar molecules)?
This ink extract was successfully soluble in another study done in Japan so we used a solvent just as polar and we were unsuccessful. This could have been due to the age of our cuttlefish ink I think it was around 4 years old and was already opened. Another idea for the solvent could be PBS which is another polar solvent.
I do not know if they were flawed or not, but they were done by different people. The professor herself did the trials with the ethanol with the negative control which is where we got the best results. However, this does not mean the data with the DMSO as the negative control is flawed because we saw some of the same trends in one of the graphs.
What exactly is a “Hit”? You define it by a line on the graph, but what does the line truly indicate? Why was using DSMO an accident? In other words, why does DSMO not work as a negative control? Why did you include the results of using DSMO when it was used accidentally?
The hit on the line is a point which is two standard deviations away and basically shows significant data points. In our case, they were points where our compound was actually effective against the bacteria. DMSO does not work as a negative control because we used ethanol as a solvent and we needed to test what the absorbance was of just the solvent to get a true reading of how well our compound worked based on the positive and negative controls. We chose to include this data because we felt like to be totally transparent we needed to include all the data we got.
Did you consider trying any other solvents to mix with the ink? Also, do you think this ink could have a better use for something else, or in a different form?
We did consider using DMSO instead of ethanol but we did not go through with it because we didn’t feel like there was enough time to get good data so we just got all the data we could with the ethanol. This ink could have a better use on tumors because that’s where most of our background knowledge came from and it is shown to work, demonstrated by the figure on the bottom of the poster.
What was the most interesting part of your research that you did this semester?
The most interesting part was how we tested the week old data on accident but had the best results with it! This was a week we were unable to go in the lab and the professor had tested this just for fun not really expecting anything and we got the best information from it.
What properties of the ink do you think makes this ink extract insoluble? What ideas do you have for a better solvent (ie amphipathic molecules / polar molecules)?
This ink extract was successfully soluble in another study done in Japan so we used a solvent just as polar and we were unsuccessful. This could have been due to the age of our cuttlefish ink I think it was around 4 years old and was already opened. Another idea for the solvent could be PBS which is another polar solvent.
So do you think that the trials with DMSO as the negative control could have been flawed?
I do not know if they were flawed or not, but they were done by different people. The professor herself did the trials with the ethanol with the negative control which is where we got the best results. However, this does not mean the data with the DMSO as the negative control is flawed because we saw some of the same trends in one of the graphs.
What exactly is a “Hit”? You define it by a line on the graph, but what does the line truly indicate? Why was using DSMO an accident? In other words, why does DSMO not work as a negative control? Why did you include the results of using DSMO when it was used accidentally?
The hit on the line is a point which is two standard deviations away and basically shows significant data points. In our case, they were points where our compound was actually effective against the bacteria. DMSO does not work as a negative control because we used ethanol as a solvent and we needed to test what the absorbance was of just the solvent to get a true reading of how well our compound worked based on the positive and negative controls. We chose to include this data because we felt like to be totally transparent we needed to include all the data we got.
Did you consider trying any other solvents to mix with the ink? Also, do you think this ink could have a better use for something else, or in a different form?
We did consider using DMSO instead of ethanol but we did not go through with it because we didn’t feel like there was enough time to get good data so we just got all the data we could with the ethanol. This ink could have a better use on tumors because that’s where most of our background knowledge came from and it is shown to work, demonstrated by the figure on the bottom of the poster.
Great Job Anthony! I am so glad that everything worked in your experiment well!
Thank you!! You too!!