Dear Kai,
EGCg might actually have the opposite effect to bacteria. Because many people drink this type of tea it could make bacteria easier to develop a resistance to this compound. This has not been proven of course this is just conjecture.
Dear Connor,
Great question, I was not able to explain this in the poster!
Bactericidal antibiotics completely kills the bacteria present in the cell. Bacteriostatic antibiotics only stops the growth of the bacteria in the body. Each type of antibiotic is very affective because once the bacteria stops reproducing the bacteria is not harmful to the body anymore.
Is it possible to use baseline level of ECGC at different concentrations without the bacteria to help get a better reading and remove the interference in the quantitative data?
Dear Erin,
Unfortunately even though you remove the bacteria from the wells when testing the issue still remains that the over the counter EGCg still leaves a sediment in the wells, even when straining the compound. It also still has a very dark red tint to it even in lower concentrations. Using the lab grade EGCg made the wells clear, so we could get a proper reading from the plate reader.
Dear Iz,
To determine if the compound is bacteriostatic or bactericidal you should perform a growth curve study. Monitor the increase in cell density of compound-treated cells against the non treated using a plate reader. The untreated cell growth produces a type of curve that you will compare to the curves obtained for the compound-treated plate.
Very good! I enjoyed your presentation. What made you use green tea?
Dear Brooklyn,
We chose green tea because it is enjoyed by millions of people daily, so we wanted to see if it really has any health benefits!
Would EGCg be harder for bacteria to develop resistance to than standard antibiotics?
Dear Kai,
EGCg might actually have the opposite effect to bacteria. Because many people drink this type of tea it could make bacteria easier to develop a resistance to this compound. This has not been proven of course this is just conjecture.
Excellent Presentation!! What is the difference between bacteria static and bacteria dactyl (sorry if I misspelt that)?
Dear Connor,
Great question, I was not able to explain this in the poster!
Bactericidal antibiotics completely kills the bacteria present in the cell. Bacteriostatic antibiotics only stops the growth of the bacteria in the body. Each type of antibiotic is very affective because once the bacteria stops reproducing the bacteria is not harmful to the body anymore.
Is it possible to use baseline level of ECGC at different concentrations without the bacteria to help get a better reading and remove the interference in the quantitative data?
Dear Erin,
Unfortunately even though you remove the bacteria from the wells when testing the issue still remains that the over the counter EGCg still leaves a sediment in the wells, even when straining the compound. It also still has a very dark red tint to it even in lower concentrations. Using the lab grade EGCg made the wells clear, so we could get a proper reading from the plate reader.
What type experiments could be done to better understand if the compound is either bacteria static or bacteria dactyl?
Dear Iz,
To determine if the compound is bacteriostatic or bactericidal you should perform a growth curve study. Monitor the increase in cell density of compound-treated cells against the non treated using a plate reader. The untreated cell growth produces a type of curve that you will compare to the curves obtained for the compound-treated plate.