10 thoughts on “D21B – Vanbaelinghem

    1. From our results alone, the biggest limitation is probably the fact that the highest dose did not represent a chemotherapeutic hit, but this could be due to the errors and limitations I spoke about in my presentation. Valproic acid was successful in at least decreasing the survival rate of the flies, so I do believe further testing should be conducted.

  1. How could you isolate p53-mutant larvae in order to conduct further experiments? Also, do these organisms have a similar response as we do to a mutant p53?

    1. That’s a good question! I’m not entirely sure how we would isolate the p53 mutants, but I assume we would breed them in population cages just as we did with the non-mutated larvae. p53 does function in the same way as in humans in that it induces stage specific cell death and p53 mutant larvae would be representative of cancer cells with the same mutation.

    1. The larvae survival rates were quantified after the addition of acid based on visual observations. We noticed that more pupal cases were yellow, containing a black figure that represented a dead pupa. A smaller amount of pupal cases were more transparent, indicating a larva that survived to become a fly.

  2. Did you choose valproic acid because it’s already an FDA approved drug, or were there other factors that went into choosing valproic acid?

    1. The main reason we chose valproic acid was due to previous research on breast, lung, and pancreatic cancer patients that indicated valproic acid is a pretty successful chemotherapeutic. Additionally, its function as an HDAC inhibitor makes it effective at killing cancer cells by damaging DNA and DNA repair pathways.

  3. Drosophila melanogaster larvae share many of the same characteristics as cancer cells, such as rapid cell division. They also mimic the three-dimensional and multi-cell type environment of real cancer cells.

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