11 thoughts on “D21b – McKenzie

    1. Although it is highly dependent on the type of infection, antibiotics should be prescribed sparingly. It should be noted that these drugs have highly toxic effects, and a therapeutic index should be established to determine the lowest dose that would still have antibiotic effects. Serum concentrations within patients had a concentration of Dasatinib of approximately 20 ug/mL, and the effective minimum dose for Dasatinib to be considered an antibiotic was around 5 ug/mL. However, the antibitoic ampicillin had roughly the same concentration but a much lower absorbance. So in broad terms it is much more dangerous than ampicillin, however due to an increasing amount of antibiotic resistant strains, it may be a viable option.

  1. This is really well done! Would the rate of increasing/using novel antibiotics be able to compete with the eventual bacterial resistance?

    1. This is a good question. Antibiotic resistant bacteria are actually more prevalent due to over prescription in medical practice, overuse in agriculture uses, and negligence in patient consumption. All of the factors increase the presence/likelihood of antibiotic bacteria. Identifying new/novel antibiotics will indeed be the first step in the right direction, but if these other factors are not properly addressed, selective pressure will continue to encourage the propagation of antibiotic resistant bacteria.

  2. Do you know what the effect of Tyrosine Kinase Inhibitors is on gram-positive bacteria, such as would TKI’s be able to inhibit bacterial growth?

    1. Gram positive bacteria lack a peptidoglycan layer, which actually makes them much easier to detect and eliminate within the body. TKI’s inhibit a cellular mechanism not involved with degrading/disrupting the cell wall of bacteria, so with this information in mind, it is very likely that TKI’s will have a similar effect to gram positive bacteria.

  3. I was wondering what the tyrosine inhibitors would do if administered to a living patient? Since these inhibitors are used in cancer treatment could it be assumed that treating bacterial infections with these drugs could have adverse affects on the patient?

    1. Yes absolutely, these drugs are primarily utilized to treat invasive forms of cancer. That is why it is so important to minimize these effects by lowering the dose as much as possible. Phosphorylation is a biochemical signaling mechanism that is widely used in the body, so the specificity of this drug is somewhat broad. Further research may be conducted in determining a more site specific vehicle of administration to effect target bacteria and only bacteria. This experiment used doses that were significantly lower than those observed in patients with cancer, however in vivo studies with animals need to be conducted to further assess the potential negative side effects, even with a significantly lower dose.

    1. In vitro studies are viable when addressing phenotypic experimentation. However these studies are poor representatives of living organisms. Modifications to the previous experiment will needed to support the original hypothesis. However if future experimentation at lower doses in the animal model shows similar results and minimized side effects, it is entirely possible this can be considered a viable antibiotic.

  4. While the entire experiment seems very complex, what experiment did you find the hardest to perform in the search for your results?

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