11 thoughts on “D22d – Storm

  1. I thought you did a really good job explaining the background information. I also feel that you incorporated a lot of useful research when building your hypothesis. Overall you seemed very knowledgable!

    1. Even though we didn’t test cytosine, the benefit of testing it would be that it may serve as a point of reference for the effectiveness of azacitidine as a bacterial growth inhibitor.

    1. Yes, I think azacitidine would be safe to use in mice as it is already being used in humans as a chemotherapeutic. By using it in mice, it would allow us to see the drug’s interactions specifically within an organism infected with salmonella before moving to human trials.

  2. what would you personally recommend as a solution for the lack of research on antibiotic expansion?

    1. This is a very tricky question! I would recommend incentivising antibacterial research by rewarding any who create a new FDA approved drug. Since there is low profitability in the antibiotic world, this may lure researchers back into the field. I’m not sure who would be giving out the reward, maybe the FDA themselves.

    1. I am not sure if a higher dose would make the dose’s effectiveness last longer. However, to determine this, we could incorporate different concentrations in the time kill assay I discussed under “Future Directions” and compare them to each other.

  3. What was your favorite part in conducting this experiment and which part did you find to be the most difficult in completing and receiving the clear results wanted in this lab?

    1. My favorite part in conducting this experiment was simply using the lab equipment! Even though we didn’t receive the best data for our time-kill assay, we know how to change the procedure to yield the results we are looking for. Therefore, I’d say creating a dose response curve was difficult, as it took us a few trials to obtain the curve we did.

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