7 thoughts on “D23 – Nguyen

  1. Do you have any possible explanations for the variability in your dose response experiment shown in figure 2?

    1. Hi Nicole, as you can see the percent survival decreases as the concentration decreases. This represents the radiation-protective property of Vernonia amygdalina. When we decrease the dose, there is less Vernonia amygdalina in the vial to protect Drosophila melanogaster against radiation; therefore, more flies get killed and the percent survival decreases. Given that infomation, we expected to see a decreasing trend line, but there was a trough between 12.5% and 6.25%, I think this might be due to the numbers of larvae in every vial were not consistent (the table under limitation shows the total number of larvae in each sample). I also address this problem in the future directions where we can place the same number of larvae in each vials and then we hopefully will obtain a better trend line.

      1. Also, thank you for your question! And my deep apology if you had a hard time understanding what I spoke because of my accent :).

  2. Is there anything that you would do differently, methods wise if you were going to perform the experiment again?

    1. Hi Sophia, at this moment, I would retest Vernonia amygdalina at different concentrations to determine if Vernonia amygdalina is actually radiation-protective. In addition, to better control the internal validity of the experiment, I will also have vials with same number of larvae. After that, I would test it on mutated versions of Drosophila melanogaster; this would more validly for me to generalize our findings to cancer cells.

      Thank you for your question! And I apologize if you had a hard time understanding what I spoke :).

  3. I enjoyed your presentation! If you were to do this again, would you have used something different besides ethanol? If that had an impact on the drosophila?

    1. Hi Connor, to be honest, I would like to try another herb or plant. Because, according to record, there are a lot of plants used in treating cancer in traditional medicine. However, we don’t see much scientific research on those plants. Therefore it is interesting to try those. However, I don’t have anything in my mind at the moment. I would probably keep going with Vernonia amygdalina at the moment. Also about ethanol, I don’t really have hope about ethanol because flies respond to ethanol similarly to human response to ethanol; therefore, testing ethanol effect on Drosophila melanogaster and hoping for a chemotherapeutic effect from ethanol do not sound reasonable and reliable.

      Finally, thank you for your question! I also apologize because you definitely had a hard time understanding my accent Connor. 🙂

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