Hi, thank you! I believe it was experimental error and the doses. I do believe we need a better ratio of chloramphenicol to CBD in order to achieve the results we were looking for.
I believe the CBD oil didn’t have an effect on the bacteria because CBD doesn’t have any properties to kill bacteria on it’s own. When combined with the chloramphenicol I believe it didn’t work because we didn’t get the ratio correct.
I believe the CBD oil had such a minimal effect because it wasn’t mixed with a high enough volume of chloramphenicol which was supposed to act as the active agent in killing the bacteria. We also knew going into it that it might not work because previous research with mixing CBD and an antibiotic was tested using a gram-positive bacteria while here we used a gram-negative bacteria.
If we test the compound at other ratios and we still don’t yield promising results then it would prove that we don’t have a “hit” which means we’d have to abandoned the idea of the mixture being a potential antibiotic.
One question that I have is even though you were not able to get the results you wanted in these expierments, but after further testing and when you do get the results for your hypotheses do you think that the combination of CBD and Chloramphenicol could be used on other infections? What type of testing would need to be done to find this?
There is a possibility this combination could be used to treat other bacterial infections. In order to test this we would start by testing it on another gram-negative bacteria, like Salmonella Enterica. After this we could test the compound on gram-positive bacteria to see if it yields the same results.
hi great presentation, what are some factors do you think led to the compound not producing the desired results?
Hi, thank you! I believe it was experimental error and the doses. I do believe we need a better ratio of chloramphenicol to CBD in order to achieve the results we were looking for.
Your presentation was really long, why did you think the CBD oil didn’t have an affect on the bacteria?
I believe the CBD oil didn’t have an effect on the bacteria because CBD doesn’t have any properties to kill bacteria on it’s own. When combined with the chloramphenicol I believe it didn’t work because we didn’t get the ratio correct.
Why do you think the CBD oil had such a minimal effect?
I believe the CBD oil had such a minimal effect because it wasn’t mixed with a high enough volume of chloramphenicol which was supposed to act as the active agent in killing the bacteria. We also knew going into it that it might not work because previous research with mixing CBD and an antibiotic was tested using a gram-positive bacteria while here we used a gram-negative bacteria.
What would you do if no other ratios work and produce the desired results?
If we test the compound at other ratios and we still don’t yield promising results then it would prove that we don’t have a “hit” which means we’d have to abandoned the idea of the mixture being a potential antibiotic.
One question that I have is even though you were not able to get the results you wanted in these expierments, but after further testing and when you do get the results for your hypotheses do you think that the combination of CBD and Chloramphenicol could be used on other infections? What type of testing would need to be done to find this?
There is a possibility this combination could be used to treat other bacterial infections. In order to test this we would start by testing it on another gram-negative bacteria, like Salmonella Enterica. After this we could test the compound on gram-positive bacteria to see if it yields the same results.