Thank you! We chose DMSO as our negative control because it has no effect on our compound. So in our negative control experiment, we could get a reliable percentage of Drosophila survival from the radiation alone by using DMSO. We used Colchicine as our positive control because this compound caused a 0% survival rate, which is what we want for our positive control experiment.
Absolutely. We were disappointed when we ran out of Omeprazole because even though the results for Pantoprazole were proving to be more of a potential chemotherapeutic than Omeprazole, both compounds were acting like chemotherapeutics so it would have been nice to test it with non-irradiated and irradiated Drosophila as well.
Since you noticed a difference in effectiveness between omeprazole and pantoprazole, do you think they each could be used for different types of tumors that may or may not need more of the effects caused by radiation and still be successful despite their differences?
Thats a good question, I think ultimately it wouldn’t matter because Omeprazole and Pantoprazole are both proton pump inhibitors. They would both act the same way in any sort of tumor; they both inhibit the function of the proton pumps in any cancerous cell, just that Pantoprazole might work more efficiently than Omeprazole.
Good presentation! Would you want to redo this experiment testing different concentrations of pantoprazole to see if that significantly impacts the survival rate? Would running an experiment like this be useful?
Thank you! Actually we did do this in our serial dilutions experiment. For each dilution that we made a different concentration amount of our compound went into each of our 3 dilutions. So we did test the different concentrations of Pantoprazole, and this test they proved to be very useful. Because of the results from this experiment, we were able to see that our compound Pantoprazole follows the same trend of results as a chemotherapeutic would!
Hi Mamoona! DMSO stands for Dimethyl Sulfoxide and it is often used as a negative control solvent. We used it as a negative control, not as a positive control, because it has no effect on our compound. So in our negative control experiment, we could get a reliable percentage of Drosophila survival from the radiation alone by using DMSO.
Great presentation! What was the reasoning you chose DMSO and Colchicine for your positive and negative controls?
Thank you! We chose DMSO as our negative control because it has no effect on our compound. So in our negative control experiment, we could get a reliable percentage of Drosophila survival from the radiation alone by using DMSO. We used Colchicine as our positive control because this compound caused a 0% survival rate, which is what we want for our positive control experiment.
Would you want to redo this experiment because you ran out of the one product?
Absolutely. We were disappointed when we ran out of Omeprazole because even though the results for Pantoprazole were proving to be more of a potential chemotherapeutic than Omeprazole, both compounds were acting like chemotherapeutics so it would have been nice to test it with non-irradiated and irradiated Drosophila as well.
Since you noticed a difference in effectiveness between omeprazole and pantoprazole, do you think they each could be used for different types of tumors that may or may not need more of the effects caused by radiation and still be successful despite their differences?
Thats a good question, I think ultimately it wouldn’t matter because Omeprazole and Pantoprazole are both proton pump inhibitors. They would both act the same way in any sort of tumor; they both inhibit the function of the proton pumps in any cancerous cell, just that Pantoprazole might work more efficiently than Omeprazole.
Good presentation! Would you want to redo this experiment testing different concentrations of pantoprazole to see if that significantly impacts the survival rate? Would running an experiment like this be useful?
Thank you! Actually we did do this in our serial dilutions experiment. For each dilution that we made a different concentration amount of our compound went into each of our 3 dilutions. So we did test the different concentrations of Pantoprazole, and this test they proved to be very useful. Because of the results from this experiment, we were able to see that our compound Pantoprazole follows the same trend of results as a chemotherapeutic would!
Hi! I was wonder what is DMSO and why was it used as a positive control?
Hi Mamoona! DMSO stands for Dimethyl Sulfoxide and it is often used as a negative control solvent. We used it as a negative control, not as a positive control, because it has no effect on our compound. So in our negative control experiment, we could get a reliable percentage of Drosophila survival from the radiation alone by using DMSO.