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9 thoughts on “D2A – Capolungo”
What was the most challenging aspect of your research?
I personally thought the most challenging part of the research was quantifying survival rates, We had to count 50-150 pupa in the vials where we tested our compounds, it doesn’t seem too hard until you’ve been doing it for a long time.
you said you reached 6.25%, what is the reason for this exact percentage of the compound? and why didn’t you go lower?
and did this percentage provide the expected results?
We got to 6.25% because that’s how we were supposed to set up our dilution series. We started at 100% then went to 50%, then to 25%, then to 12.5% and then finally to 6.25%. This is the percent ratio with our compound compared to DMSO in a solution which we then added to our vials for research. We tested each dilution multiple time and I personally didn’t expect the 6.25% to be considered a hit I was thinking 100% or 50% dilution of our compound would have created a hit instead of the 6.25%.
How did you determine if the drug worked or not?
We can tell if the drug had an affect if the survival rate was less than 50%, what was considered at hit would be a 14.05% survival rate.
The drug worked or was somewhat effective if there was a survival rate less than 50% because the radiation kills roughly 50%. A hit is considered if the survival rate was 14.05% or less.
How does the chemical selection go, ie. how did you know which compounds showed promise and that you wanted to use them?
We picked these compounds that had similar chemical structures to known chemotherapeutics, That show promise because the structures were similar.