9 thoughts on “D34 – Kunapuli

  1. What was the most interesting/favorite procedure you carried out during this lab? It looks like it was really interesting to work with Salmonella typhimurium!

    1. I think carrying out the Dose Response procedures was the most fun. Working with S. Typhimurium is definitely interesting.

    1. Using Piper Guineense requires crushing the seeds and creating our own stock solution before testing its max dose and dose responses. This leaves a lot of room for human error. Linalool is an active compound in Piper Guineense that is professionally derived in liquid form which allows us to test without having to rely on our own accuracy. Testing both also allows us to find out if it’s the seeds as a whole or if it’s a specific active compound that is antimicrobial.

  2. Great job! Was there a dose response curve performed for pleuromutilin? If so, was there an observed cutoff in its effectiveness?

    1. Woops! Sorry! Posted the wrong question!! Here is your question: Great job! How did you choose which active compounds to test here?

      1. Piper Guineense is common in herbal medicines in West Africa, so we wanted to test its antimicrobial properties that were proposed or tested in other studies we found. The Linalool was a bit easier to find than the Piper Guineense seeds so we ended up testing both.

  3. My question is what made the ring around Linalool abnormally shaped? Along with that, what does a normal ring look like?

    1. If you look at the picture of our plates, we know that Ampicillin works against bacteria, so we used it as a positive control, so the ring around that well is almost a perfect circle and it is almost clear (which indicates it got rid of the bacteria). We are unsure why the ring around Linalool was abnormally shaped. We think it has something to do with the effectiveness of Linalool at lower doses and how the Linalool actually affects the bacteria (does it penetrate the bacteria, does it inject something into the bacteria, does it inhibit some proteins on the bacteria?). In the future we would like to explore these questions further.

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