10 thoughts on “D36 – Knoshaug

    1. I would get dried papaya leaves and perform my own ethanol extraction instead of using the liquid dietary supplement and would do the same with papaya seeds and potentially the fruit to see if our results are different when approaching from this angle.

  1. Cool research! What is a Mueller-Hinson plate and what is it used for in the context of your research?

    1. A Mueller-Hinton plate is a petri dish with agar (basically sugar) that feeds a sample of Salmonella bacteria spread across the plate surface. Wells are made in the agar and our drugs were administered to these wells to see if the bacteria would be pushed back, making a zone of inhibition. The larger the radius extending from the well with the drug, the more of an antibiotic effect the drug must have had. Neither of our drugs had a noticeable zone of inhibition, though, meaning the Salmonella grew unaffected.

  2. What is a “zone of inhibition,” which you mentioned when talking about the Mueller-Hinton plate results, and how does it form during your experiment?

    1. A Mueller-Hinton plate is a petri dish with agar (basically sugar) that feeds a sample of Salmonella bacteria spread across the plate surface. Wells are made in the agar and our drugs were administered to these wells to see if the bacteria would be pushed back, making a zone of inhibition. The larger the radius extending from the well with the drug, the more of an antibiotic effect the drug must have had. Neither of our drugs had a noticeable zone of inhibition, though, meaning the Salmonella grew unaffected.

    1. The Lycopene was not soluble in the media the bacteria lives in, and so the compound could not have been tested without the ethanol extraction.

  3. What was your reasoning behind using water as a negative control but ethanol in your extraction method for your compound?

    1. The leaf extract was soluble in water and so a different solvent system/negative control did not need to be found for this drug. The Lycopene needed to be extracted because of its insolubility, a process most commonly done using ethanol. The leaf extract negative control was water, though could have been a glycerin/citric acid mixture as these were the other two ingredients in the extract we used. Future experiments should use the glycerin/citric acid negative control rather than water or at least alongside water if the leaf extract is to be investigated again.

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