10 thoughts on “D40 – Fouts-Hyatt

    1. We had a 1:2 dilution series, to see which concentration of melittin would be most effective, and we placed around 50 larvae into each vial that we tested. We also did two per series and two per dilutions.

    1. We found that melittin may be a poison because it had a very low therapeutic index meaning it was too toxic to be used as a chemotherapeutic. The compound, melittin, is actually the main compound in honey bee venom so we knew it was going to be toxic, we just didn’t know the severity of it. You can also see on the graphs that it acted as a poison in several vials by looking at the percent survival.

  1. Good work on your presentation! I am wondering how the level of significance necessary for a ‘hit’ was determined? Is this a standard level for this kind of research? Was it specific to your experiment?

    1. We determined for our lab that a hit was based on our negative control which was DMSO, minus two standard deviations from the mean. Yes, the requirements for hits are the same in all Drug Discovery Labs because this is where you can identify if your compound worked as a chemotherapeutic or not. It was not just specific to my experiment.

  2. Do you know of any chemotherapies that would work synergistically with your compound?

    1. I do not know of any as of now, but in the future, we would hope to find a compound that would work in combination with melittin and radiation that would produce a better therapeutic index and a lower percent survival (but keeping healthy cells alive).

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