13 thoughts on “D44 – Angel

  1. Since your results were mostly inconclusive, would there be a way to go back to old experiments to determine the reason why they were inconclusive such as possible contamination?

    1. Unfortunately no, we do have our speculations though. We think there may have been an issue with our sample that we extracted “expiring” or that there was human error on our part for the dilutions.

  2. In your background, you mention that mugwart is often used to prevent food spoilage. If this is the case, why do you think there was no zone of inhibition around the compounds that you tested? Is is possible that there could be some other compound(s) in mugwart that is/are responsible for its antimicrobial properties?

    1. Yes, we think that there is either some other compound entirely that is responsbile for the previously tested properties or that there is a missing component from our expeirment ithat is present in these conditions.

  3. Could you please explain what causes the zone of inhibition? I caught that an effective bactericide produces a zone of inhibition, but I am curious as to why it does.

    1. Yes! So basically when we put a coumpound into the hole in the agar plate, it will diffuse to the surrounding areas. If the compund is an antibiotic, then there wil be no bacteria growth in that diffusion area (hence the zone of inhibitions)

  4. Why do you think there was variability in the hit results of the max dose experiment? Would you consider trying a lower percentage of ethanol (to help with the negative control issues) and repeating the dose response curve?

    1. We did substitute the ethanol with water as a negative control for later experipemtns. But there was no indication that it was causing problems during our max dose experiment.

  5. Why do you think there was so much variability in the max dose hit results? Would you consider repeating the dose response curve with a lower concentration of ethanol?

    1. Yes, my group members and I talked so much about how we feel unsure about the mixed data and wish we had another semester to solidify our findings.

    2. WE beleive that altering slight components of the compunds may make them more effective agains t salmonella, mianly becasue there was a lot of inconclusive data.

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