12 thoughts on “D4B – Sanchez

  1. Very nice video! Has this ever been tested on humans and what are the potential side effects from this treatment?

    1. Hi Isabella, great question! So astragalus extract hasn’t been tested on humans as a potential chemotherapy so the potential side effects aren’t known. But a human cancer cell line could potentially be a great model organism for future testing of astragalus where the effectiveness of the compound could be further determined.

    1. Hi Noah, thank you for your question. I believe that the most effective treatment using astragalus would be to couple it with an efflux pump inhibitor. In our study, we saw that even with the highest dose, astragalus still produced a percent survival not indicative of an effective chemotherapy. With an efflux pump inhibitor, the concentration of the drug in the cells would increase which would hopefully increase the effectiveness of the compound and decreasing the percent survival of the tumor cells.

    1. Hi Jaedyn, thank you for your question. In our first experiment, we chose the highest dose to see if our compound at its max dose would be effective at killing the Drosphila melanogaster. In our second experiment, we chose lower doses that are typical in a dosing series with our first dose being a 1:100 dilution of our stock compound. Lower doses are tested to see if the compound is effective at killing the Drosphila melanogaster at lower doses and is used to find the ED50 or effective dose.

  2. Are there other compounds in this family that could be more effective than this one? And what would be some of the benefits of testing this particular compound on vertebrate species that we would not see in Drosophila?

    1. Hi Ellie, thank you for your question. I am unaware of promising research specifically studying other Chinese medicinal compounds similar to astragalus but looking into other compounds that use other mechanisms could be a promising future area of research. For your second question, vertebrates such as mice are very different from Drosophila. For example, the reason why clinical trials are so important for any drug is that a compound can show extremely different effectiveness and side effects in humans than in mice or whatever species the drug was tested on in pre-clinical trials. This same logic is used here when thinking that Drosophila might just be the wrong model organism for astragalus and we might see different (hopefully promising) results if it were to be tested in a different organism such as mice or other vertebrate species.

  3. Why did you hypothesize that less than 50% of the drosophila would survive when given the drug? Was this figure chosen because of some significance for how cancer cell populations grow?

    1. Hi Thomas, thank you for your question. We hypothesized that less than 50% of the Drosophila would survive based on multiple articles determining astragalus as being effective when treating lung and breast cancer. Since 50% of the Drosophila are killed by radiation alone, an effective chemotherapy would show a percent survival less than 50%.

  4. What did you base your hypothesis off of and why do you think your results were inconsistent with the hypothesis?

    1. Hi Analise, great question. Astragalus extract has been shown in multiple studies to be an effective treatment for lung and breast cancer. Past successful studies coupled with the fact that the compound was identified as a hit in a previous semster, led us to believe that astragalus in combination with radiation would demonstrate a percent survival less than 50%.

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