12 thoughts on “D4c – Kapp

  1. I really liked your background information. It was very interesting and seems very relevant to your experiment. I also felt like you explained your materials and methods really well. Overall you seemed very knowledgable!

  2. How could you ensure that a concentration of Cisplatin would not be toxic when taken in clinical trials?

    1. In order to be relatively certain that it is not a toxic concentration, it must be outside of two standard deviations of the negative control (DMSO). It can’t be 100% certain that it is not toxic at this point, but it is considered a HIT, which is a promising result and is due for further testing.

  3. how is a ‘safe’ concentration determined? how can a known safe concentration be implemented nationwide

    1. A safe concentration can be determined if it is outside of two standard deviations of the negative control. It is not necessarily certain, but subject to more testing. A result that is similar in concentration to ampicillin (the positive control), is deemed safe. Same will apply nationwide as ampicillin is a nationally recognized antibiotic.

    1. A safe concentration can be determined if it is outside of two standard deviations of the negative control.

    1. A safe concentration can be determined if it is outside of two standard deviations of the negative control.

  4. Our hypothesis predicted that the cisplatin would be bactericidal, and we never ran a time series to determine whether or not it is bactericidal or bacteriostatic. Further testing would be required in order to determine that. But Cisplatin did prove to at least compare in absorbance value with the ampicillin absorbance.

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