I really liked your background information. It was very interesting and seems very relevant to your experiment. I also felt like you explained your materials and methods really well. Overall you seemed very knowledgable!
In order to be relatively certain that it is not a toxic concentration, it must be outside of two standard deviations of the negative control (DMSO). It can’t be 100% certain that it is not toxic at this point, but it is considered a HIT, which is a promising result and is due for further testing.
A safe concentration can be determined if it is outside of two standard deviations of the negative control. It is not necessarily certain, but subject to more testing. A result that is similar in concentration to ampicillin (the positive control), is deemed safe. Same will apply nationwide as ampicillin is a nationally recognized antibiotic.
Our hypothesis predicted that the cisplatin would be bactericidal, and we never ran a time series to determine whether or not it is bactericidal or bacteriostatic. Further testing would be required in order to determine that. But Cisplatin did prove to at least compare in absorbance value with the ampicillin absorbance.
I really liked your background information. It was very interesting and seems very relevant to your experiment. I also felt like you explained your materials and methods really well. Overall you seemed very knowledgable!
Thanks so much, thanks for listening!
How could you ensure that a concentration of Cisplatin would not be toxic when taken in clinical trials?
In order to be relatively certain that it is not a toxic concentration, it must be outside of two standard deviations of the negative control (DMSO). It can’t be 100% certain that it is not toxic at this point, but it is considered a HIT, which is a promising result and is due for further testing.
how is a ‘safe’ concentration determined? how can a known safe concentration be implemented nationwide
A safe concentration can be determined if it is outside of two standard deviations of the negative control. It is not necessarily certain, but subject to more testing. A result that is similar in concentration to ampicillin (the positive control), is deemed safe. Same will apply nationwide as ampicillin is a nationally recognized antibiotic.
What concentration determines if the substance is poisonous or not?
A safe concentration can be determined if it is outside of two standard deviations of the negative control.
How can you determine whether or not Cisplatin would be toxic to normal cells?
A safe concentration can be determined if it is outside of two standard deviations of the negative control.
I may have missed it but was your hypothesis supported by your results?
Our hypothesis predicted that the cisplatin would be bactericidal, and we never ran a time series to determine whether or not it is bactericidal or bacteriostatic. Further testing would be required in order to determine that. But Cisplatin did prove to at least compare in absorbance value with the ampicillin absorbance.