18 thoughts on “D52 – Koskella

  1. Hey do you think that the highest survival rates were at the lowest and highest ends of the U-shaped curve?

    1. Highest survival rates observed in our compound were, surprisingly, in the higher concentration doses, meaning it would be on the left of the dose response curve (U-shaped curve).

    1. I was actually absent when my group choose this compound to be tested, but I liked this compound because snake venom is often known to kill living things and I found it fascinating that snake venom played an important role in the medical world.

    1. For this lab, we were able to choose our own compound to test whether it could be a possible chemotherapeutic.

  2. What specifically would you do to increase the accuracy/precision of the dosing series if you reproduced the experiment?

    1. For this lab, we weren’t able to get the necessary data to accurately examine the results as one of vials was given non-irradiated larvae when it was supposed to have radiated data. Because of this, we were short one vial for data collection. For future experiments, we would produce more trials of the dosing series, from only 2 trials to 8 or more. This would allow more data accumulation and accuracy of each dosing series’ results.

  3. Do you think your only trial was accurate, or do you think extra trials would produce different data?

    1. We actually had 6 trials total, 3 with radiated larvae and 3 with non-irradiated larvae, however, our data was not accurate as we had a misunderstanding in the lab as one of the vials were given larvae that were non-irradiated instead of radiated larvae. This resulted in less accuracy of the dosing series data that lacked one dosing series data. Extra trials would definitely lead to different data as data would be accumulated more results rather than a small population of data that are not accurate.

  4. What provoked you to study Crotalus Atrox venom? Do you think if you repeated this experiment a couple of times, you would come up with the same conclusions?

    1. I was absent when my group choose this compound to test, however, if I was to give a reason, it would be because I found it interesting that snake venom is not all bad like we all thought it was, but rather, it had a side to it where it could be potentially be saving lives instead of killing. I think repeated experiments would come up with different conclusions as there were mess-ups during the lab as we didn’t produce a vial for one of the dosing series and instead made that missing vial a vial for an already group of dosing series. This led to the dosing series with the missing vial to have inaccurate data due to the small number of vials to collect data from.

    1. Efflux pumps are activated when toxic substances are found in cells of eukaryotic cells. Signals are sent within the cell to tell the pump the toxic substances present in the cell. In our lab, the activation of the pumps occurred in high dosing concentration series. To address efflux pump activation, we could give the ‘cancer cells’ efflux pump inhibitors to prevent the pumping of our compound outside the cell. This would allow our compound to play its role in the cell, resulting in the reduction of the Drosophila Melanogaster.

  5. What would be some challenges of translating this chemotherapy to humans, as I’m sure there are many differences in testing on Drosophila Melanogaster and actual people?

    1. Although Drosophila Melanogaster have 75% of cancer causing genes, they are, like you said, still much different than actual people. To start, snake venom, by itself is a toxin so not only would they kill cancerous cells, but also healthy cells. Research done involving snake venom in cancer patients do not just use snake venom, specific toxins within the venom are extracted and then used accordingly to the needed uses. Since it is a toxin, extreme caution is needed because it can kill as easily as it can be designed to save.

    1. I’m not sure what you are asking, I think it’s about the graphs? The x-axis is the dosing series. From left to right, 20, 10, 5, 2.5 (all ug/mL), class + control (colchicine), class – control (DMSO), our + control, and – control. Y-axis is the percent survival. The experiments we tested are the effects of our compound with different dosing series on Drosophila Melanogaster listed earlier: 20, 10, 5, 2.5 (all ug/mL).

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