10 thoughts on “D56 – Arthur

  1. Good job Liv! It seems like the dosing series’ results don’t follow a constant trend. Any thoughts as too why?

    1. My partners and I thought that we could’ve missed the necessary dose and in our future directions our dose interval would have smaller intervals, or that our compound does not have a concentration interval that works synergistically with radiation therapy.

  2. Great presentation! It was very enjoyable to listen to, and I’m grateful that I was able to learn more about the research you conducted this semester! In the future directions section, you mentioned that a re-evaluation of the compound’s efficacy could take place by using a larger quantity of vials. Why use a larger quantity of vials?

    1. We would increase the vials to have a larger sample size of Drosophila melanogaster and collect data to have a more precise dose response curve and lead to a more conclusive result.

  3. In the future experiment section, you mention that you could increase the dosage of the compound, phospholipase A2, to see if there will be a greater effect. How would you ensure/test that the dosage of phospholipase A2 won’t be so high that it will start harming the organism as a whole if integrated as a chemotherapeutic?

    1. To test the dosages you perform both a dose response curve and a max dose response curve, and look at the percent survivals to adjust the toxicity of the compound.

  4. How long did it take for most of the organisms to develop and did they all hatch at the same time or was is spread out?

    1. Takes ten days to become a full adult and roughly five days to become the third instar larva which is our model tumor, and we would try and keep a similar age. After we collect embryos we would culture them until the third instar larva could be collected.

    1. Drosophila melanogaster populations can grow exponentially, in a laboratory setting, in days due to their quick reproductive pathways: wild-type flies produce large populations for testing. This results in high levels of mutated cells, similar to tumors.

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