12 thoughts on “D60 – Downing

    1. Luteolin was dissolved in DMSO in order to put the compound in the food that the model organism ate, which makes DMSO our negative control.

    1. 2500 rad kills about 50% of the population, and that population is then used to conduct the research for potential chemotherapeutics.

  1. what is the purpose of irradiating the larvae before treating them with the positive, negative, and luteolin when the larvae development is comparable to the development of cancer cells?

    1. Irradiating the larvae both directly and indirectly damages the DNA of the larvae, which then can be further affected by the various compounds. The aim of the research done was to determine the effects of Luteolin in combination with radiation, so the larvae were irradiated and then treated with a compound.

  2. You mentioned that Luteolin has cytotoxic effects. Do you know what these cytotoxic effects are?

    1. Luteolin shows potential in inhibiting angiogenesis, which is when cells call on blood vessels to give them the nutrients they need to survive. By inhibiting angiogenesis, the cancer cell is cut off from nutrients and cannot survive.

    1. Because the positive control is a known, toxic chemotherapy, we would expect a large percentage of the larvae to die. The negative control has no chemotherapeutic effects, so we would expect a 50% survival rate due to the irradiation of the flies. A more significant positive and negative control would show the more drastic difference in percent survival between the two compounds.

    1. We were only able to conduct one experiment with two vials per compound, so our average percent survival between trials is not very accurate. We also had unusual positive and negative control data which may have made the results collected for the 4 doses of Luteolin less significant.

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