17 thoughts on “D69 – Diaz

  1. Regarding the future research…does that mean that melittin is difficult to use in the lab setting because of cell death? Would it be worth researching further despite this indication of melittin leading to cell death?

    1. Great question. Melittin is not necessarily difficult to use in the lab setting; we know the concentrations of this compound and understand what setting it can be soluble in so we can administer the compound – within Drosophila Melanogaster. We would want to see Melittin actually induce more cell death as this indicates Melittin as a potential chemotherapy.

  2. Great job on your presentation! Do you think preforming this experiment multiple times would decrease your standard deviation and lead to more clear results?

    1. Thank you! Yes, that would be our goal in repeating trials with these same doses, we would be able to further analyze the results at these doses.

  3. If you were to further these trials, would you want to do so with the intention of your compound working with a chemotherapy, radiation therapy, or alone?

    1. Great question. Further trials with a known chemotherapy (known as a positive control in our lab) would be beneficial in comparing our compound with the results of this known chemotherapy. In terms of radiation, further trials without/with radiation applied synergistically would be beneficial in determining the efficacy of this compound when it is trialed in these different conditions. To answer your question, if I were to further these trials I would include trials with a known chemotherapy, radiation therapy, and the compound alone.

  4. I really enjoyed your poster! Do you think preforming this process multiple times would decrease your standard deviation and make your results more clear or would you move forward with experimentation?

    1. Thank you! I think it is valuable in performing this process multiple times to showcase more clear results. However, I would rather perform experimentation with a higher concentration of Melittin.

    2. I think it is valuable in performing this process multiple times to showcase more clear results. However, I would rather perform experimentation with a higher concentration of Melittin.

    1. Further trials of Melittin at different doses with multiple sets of vials would need to be performed to determine if our compound can be administered as a chemotherapeutic agent or used to enhance/protect the use of administrating radiation within these cells.

  5. Do you hypothesize that melittin has the possibility of being a radiation protector? It seems that when radiation is induced, the percent survival also had a increase with higher dilutions.

    1. Great find, we found that melittin may have properties that induce more cell death without being exposed to radiation: a possible radioactive protector; however, this is not conclusive as this value did not have an average that was -2 standard deviation. However, there is literature that explores how melittin may in fact a possible radiation protector!

  6. I really enjoyed the presentation! How do you think it would have changed if you had repeated the process?

    1. Thank you! If we were to repeat this process with a different dosage series, one that has a higher concentration of melittin, for example, we would be able to determine if Melittin can induce a higher percentage of cell death at a higher concentration. If we were to repeat this process with the same dosage series; we would be able to analyze if our first experiment is inconclusive or if it relatively produces the same numbers.

  7. Why don’t you think melittin worked to kill larvae by inducing cell lysis? What differs from causing single cell lysis that you mention vs an entire organism that keeps the flies alive?

    1. While Melittin may have induced cell lysis within the larvae, percent survival was not low enough to be considered a potential chemotherapeutic agent. If Melittin influences the regulation of the cell cycle, metastasis, and angiogenesis at a high enough level within the cell this would result in clear cell death causing the entire organism to be unable to become adult fruit flies.

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