This is a very good question. My understanding is that there are seemingly limitless options for creating novel peptides such that we could keep synthesizing different peptides to combat different antibiotic resistant strains of bacteria. This is also very new research such that these options have not been explored as much as other options so they may yield more return in research investments.
Thank you for your question. Likely our conclusion would have been similar given that our data all showed little effect on Salmonella growth. However, we likely could have more conclusively dictated whether the peptide was an antibiotic or not with replication of our method trials.
How do you think you could modify your peptide to make it more effective? Would this be possible, or would you need to look for a new option altogether?
Thank you fro your question. If I was to test other peptide sequences, I would test a peptide sequence that was found from an animal that had previously shown promise at acting as an antibiotic as these were the original peptides studied in the research we referenced.
Would you consider it to be a worthwhile future direction to repeat these experiments without the experiment/human errors made during these experiments you conducted in order to get a conclusion that can be more reliable?
Thank you for your question. I feel that might be a worthwhile investment but even with the data we recovered, the compound did not even have noticeable effects at much higher concentrations than are possible in the human body so I feel that time and effort would be better spent studying different peptide compounds.
Thanks so much for your question. Every peptide sequence will interact differently with itself and other compounds based on the properties of each amino acid as each AA could be positively or negatively charged, polar or non polar, and these different interactions will (hopefully) lead to different effects of the peptides on the Salmonella growth.
Why do you think peptides are a good option for future antibiotics?
This is a very good question. My understanding is that there are seemingly limitless options for creating novel peptides such that we could keep synthesizing different peptides to combat different antibiotic resistant strains of bacteria. This is also very new research such that these options have not been explored as much as other options so they may yield more return in research investments.
How would you conclusion be different if you had more time for experiments?
Thank you for your question. Likely our conclusion would have been similar given that our data all showed little effect on Salmonella growth. However, we likely could have more conclusively dictated whether the peptide was an antibiotic or not with replication of our method trials.
How do you think you could modify your peptide to make it more effective? Would this be possible, or would you need to look for a new option altogether?
Thank you fro your question. If I was to test other peptide sequences, I would test a peptide sequence that was found from an animal that had previously shown promise at acting as an antibiotic as these were the original peptides studied in the research we referenced.
Would you consider it to be a worthwhile future direction to repeat these experiments without the experiment/human errors made during these experiments you conducted in order to get a conclusion that can be more reliable?
Thank you for your question. I feel that might be a worthwhile investment but even with the data we recovered, the compound did not even have noticeable effects at much higher concentrations than are possible in the human body so I feel that time and effort would be better spent studying different peptide compounds.
Why is it important to test similarities/differences between different peptides?
Thanks so much for your question. Every peptide sequence will interact differently with itself and other compounds based on the properties of each amino acid as each AA could be positively or negatively charged, polar or non polar, and these different interactions will (hopefully) lead to different effects of the peptides on the Salmonella growth.