Have you read other papers that use different percentages of DMSO and seen what the toxicity looks like there/is there a reason you would go from 100% down to 20 in future directions?
The reason we started with a 100% DMSO is because your compound was said to be soluble in DMSO, and because we got such a small amount, that we had to use all of it to make your stock. We weren’t sure if viriditoxin is soluble in 20% DMSO (because it’s mostly water you’re diluting in) and we didn’t want to risk it not dissolving and having to reorder the compound. If we had a greater amount of viriditoxin powder, we’d start off with the 20% DMSO.
We performed series dilutions to try and find a dose response curve with the concentration of viriditoxin to determine what concentration is most effective while balancing the amt of the drug we can put in a human.
If you are decreasing the percentage of DMSO in the future to 20% and you have to reduce the veridatoxin as well, is it proportional or will you not? And if reducd veridatoxin is less effective, how will you know you used enough to be effective.
I’m not sure it would be exactly proportional. That’s exactly what we would need further experimentation to understand. the agar diffusion test sorted out all concerns about our compound’s effectiveness, so the next steps wouldn’t really be finding better solvents – it would be modifying our compound through medicinal chemistry or looking at similar compounds or other active ingredients. we also had to keep in mind the max dose we can in good conscious put into another human being and it still work.
What outcome would you expect to see if you were to preform your future directions and do the entire experiment with Viriditoxin diluted in 20% DMSO instead of 100% DMSO?
we’d first have to see if it dissolves in the 20% DMSO – if it doesn’t we can gradually increase the concentration of DMSO. But this is all assuming we have more viriditoxin to work with. then the next steps from there would be modifying our compound through medicinal chemistry or looking at similar compounds or other active ingredients.
Have you read other papers that use different percentages of DMSO and seen what the toxicity looks like there/is there a reason you would go from 100% down to 20 in future directions?
The reason we started with a 100% DMSO is because your compound was said to be soluble in DMSO, and because we got such a small amount, that we had to use all of it to make your stock. We weren’t sure if viriditoxin is soluble in 20% DMSO (because it’s mostly water you’re diluting in) and we didn’t want to risk it not dissolving and having to reorder the compound. If we had a greater amount of viriditoxin powder, we’d start off with the 20% DMSO.
Why did you exactly perform dilution? How does it help proceed the next step of the experiment?
We performed series dilutions to try and find a dose response curve with the concentration of viriditoxin to determine what concentration is most effective while balancing the amt of the drug we can put in a human.
Why did you exactly perform serial dilution? How does it help proceed the next step of the experiment?
Why did you perform serial dilution experiment? How does it help you procced the next step of the experiment?
If you are decreasing the percentage of DMSO in the future to 20% and you have to reduce the veridatoxin as well, is it proportional or will you not? And if reducd veridatoxin is less effective, how will you know you used enough to be effective.
I’m not sure it would be exactly proportional. That’s exactly what we would need further experimentation to understand. the agar diffusion test sorted out all concerns about our compound’s effectiveness, so the next steps wouldn’t really be finding better solvents – it would be modifying our compound through medicinal chemistry or looking at similar compounds or other active ingredients. we also had to keep in mind the max dose we can in good conscious put into another human being and it still work.
What outcome would you expect to see if you were to preform your future directions and do the entire experiment with Viriditoxin diluted in 20% DMSO instead of 100% DMSO?
we’d first have to see if it dissolves in the 20% DMSO – if it doesn’t we can gradually increase the concentration of DMSO. But this is all assuming we have more viriditoxin to work with. then the next steps from there would be modifying our compound through medicinal chemistry or looking at similar compounds or other active ingredients.