10 thoughts on “D72 – Enriquez

    1. Yes! I think that additional trials could show more conclusive results of flufenamic acid as a chemotherapy given that the dosages produced a hit in some of the trials.

  1. If you did use Drosophila melanogaster with the Chk1 mutation, would you need less time to reach a result given the potential of more hits, or would you still need the same amount of time for the experiments in total?

    1. I think it would still be the same time since we have to let the larvae incubate for at least 7 days to give them the maximum amount of time to develop into flies before we quantify so we can get the most accurate results

  2. You likely explained this, but I was confused on this portion. Why was the hit threshold 54% and what does that mean for Melanogaster cell survival?

    1. The threshold was below 54% survival to be a hit because that was 2 standard deviations from the average survival of negative control flies!

    1. They have a pretty similar genome to us so their response to drug may be similar to how a cancer cell, which are our cells, would respond. We also test them in their 3rd instar stage of development which is when their cells are dividing rapidly so it kind of models how quickly cancer cells proliferate

  3. Wonderful presentation! Were you able to see if Flufenamic acid is additive or synergistic?

    1. Yeah so the results weren’t fully conclusive because our compound was only a hit in a few of our treatment vials: without radiation in the max dose experiment and the 1:3 dilution for the dose response experiment. Bc of that we can’t determine if the effect is additive or synergistic until we can get a better and more conclusive dose response curve and do more trials to really see how Flufenamic Acid effects larvae survival on a larger scale.

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