9 thoughts on “D73 – Z Espey

    1. Thank you for asking Nolan! 37 degrees Celsius the the average human body temperature. By incubating the Salmonella Typhimurium at this temperature, we could mimic the growth of it as if it were in the human body. We were trying to get it as close as possible to a physiologically relevant environment in vitro.

    1. Good question Paige. Polyphenols are organic compounds produced by all kinds of plants. They are characterized by their phenol rings, which you can see in the image of phloroglucinol on the poster.

  1. Really great presentation! What makes your chosen molecule different from others that contain hydroxyl groups in its antimicrobial properties?

    1. Thank you for asking Solomon! It’s hard to say what makes phloroglucinol stand out when comparing to other compounds that also have hydroxyl groups. The main reason we chose phloroglucinol is because many other polyphenols—which also have hydroxyl groups—inhibited bacterial growth. It was argued in a paper (Besednova, 2020) that phloroglucinol in particular showed promise.

  2. Really great presentation! What makes your molecule different from others that contain hydroxyl groups in having antimicrobial properties?

    1. Good question Daanish! Variance could be attributed to several things. Improper vortexing of solutions, improper pipetting of solutions into the well plate, as well as damage to the well plate could cause variable absorbance values. If the Salmonella Typhimurium solution was not mixed well enough, differing concentrations of Salmonella may have been administered between wells, and therefore differing absorbance values would result. It’s also important to note that bacterial growth is not always predictable, and variance should be expected in that regard.

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