13 thoughts on “D74 – Welch

  1. You mention wanting to test the compound on P53 mutants. What about the P53 mutation could be beneficial for your experiment?

    1. Hi Vinny! Thanks for watching.
      The p53 mutation in flies makes them more susceptible to radiation therapy, whereas the wild type we used have a known resistance. Employing p53 could have yielded different results when used concomitantly with phospholipase A2.

  2. How are the p53 flies compared to the wild type flies? And if you were to do the experiment again what venom would you choose and why?

    1. Thanks for watching, Teigen.
      Wild types exhibit a known resistance to radiation, so if p53 mutated flies were used, its possible that the phospholipase A2 combined with the radiation would have yielded greater results. It is also possible it wouldn’t have, but it would be something to test.
      Initially, we had wanted to test a compound from sea anemone venom, so that’s where I would like to start. Unfortunately, we couldn’t find a way to obtain it, but there is some new research being done on it and its ability to combat cancerous cells.

    2. Wild type flies are known to have resistance to radiation therapy, but p53 flies are not. While there isn’t a guarantee, it would be interesting to see if results varied with this fly type when used with Phospholipase A2 on irradiated flies.
      If we could choose another venom, we would have used venom from the sea anemone. That was what we initially attempted, but we couldn’t find a way to obtain it. There has been some new research conducted on this compound relating to combatting cancer, so it should be explored further.

    1. Hi Katarina, thanks for watching!
      It’s a good question. Potentially nothing. Unfortunately, we didn’t have enough of our compound to test a more concentrated solution, but there’s a potential that a greater concentration could have yielded better results that were more in line with what we were hoping – a lower percent survival of flies. However, it could just be the compound itself doesn’t have any hope as a chemotherapeutic and that a greater concentration wouldn’t have accomplished anything.

    1. Hi Gallegos,
      Thanks for watching.
      If p53 mutant flies were used, there is a chance they could have been more responsive to the radiation as this type fly is shown to be more receptive than wild type flies.
      However, there is always the chance that the flies wouldn’t have done anything differently and that the compound itself was the problem.

    1. Morgane,
      Thanks for viewing!
      Grape juice agar is just the medium that was used to spread the yeast paste on for the flies to deposit their embryos. It didn’t serve a purpose other than for setting up a nice little home for the flies to drop their eggs.

  3. You mentioned that in you results you did not observe any “hits”, what do you think may have caused this in comparison to the controls used and their expected outcomes?

    1. Hi Kenneth,
      I think the fact that we didn’t have any hits for our compound could be related to the fact that our compound did not serve as a chemotherapeutic agent. Colchicine is a known chemotherapeutic that ideally should have produced a 0% survival rate (although it did not in our dilution series experiment). Regardless, it does have cytotoxic capabilities, but the lack of hits from Phospholipase A2 would indicate that it does not. Of course, the limitations detailed in the video could also be the cause for this – too low of concentration, only one experimental attempt, etc.

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