The best way to test toxicity levels are through CBC test also known as a complete blood count and through those tests we can see how much compound is in the body and see if there are any external and internal problems.
We picked commonly used dilutions that are used in other types of experiments to test concentration and those are also how doses are done in human trials.
We used dilutions that are used in multiple experiments to test concentration of compounds and proteins, and also we followed the direction of our TAs.
It would takes months to years of testing to move to human trials just to make sure the compound is not toxic and that it would work in an animal environment.
An alcohol being dissolved in an alcohol would be very soluble in the solvent and thus, the extracellular space outside of the bacterial cells. Some compounds may not be taken into a cell if the compound would be much more energetically favorable in the solvent. How confident are you on the uptake of the compound into the bacterial cells?
We are very confident in the uptake in bacterial cells. In testing we used an M9 media to suspend the bacteria in and because Salmonella has more defense to protect it against antibiotics the results showing that Terpinen killed the bacteria show that it will have good uptake in bacteria in general.
How do you plan on testing the toxicity when ingested of Terpinen-4-ol?
The best way to test toxicity levels are through CBC test also known as a complete blood count and through those tests we can see how much compound is in the body and see if there are any external and internal problems.
Did you guys choose your dosages from trial and error, or did you pick them from commonly used dosages from other experiments?
We picked commonly used dilutions that are used in other types of experiments to test concentration and those are also how doses are done in human trials.
We used dilutions that are used in multiple experiments to test concentration of compounds and proteins, and also we followed the direction of our TAs.
How long would it take for research like this to become an available option in medical treatments?
It would takes months to years of testing to move to human trials just to make sure the compound is not toxic and that it would work in an animal environment.
What is your reasoning for choosing ampicillin as a control?
Ampicillin is a very well known antibiotic that is known to kill Salmonella and so we knew it would always work.
An alcohol being dissolved in an alcohol would be very soluble in the solvent and thus, the extracellular space outside of the bacterial cells. Some compounds may not be taken into a cell if the compound would be much more energetically favorable in the solvent. How confident are you on the uptake of the compound into the bacterial cells?
We are very confident in the uptake in bacterial cells. In testing we used an M9 media to suspend the bacteria in and because Salmonella has more defense to protect it against antibiotics the results showing that Terpinen killed the bacteria show that it will have good uptake in bacteria in general.
We chose the doses through dilutions that are used in other experiments and also the advice of our TAs
Thank you all for your questions, they were great questions to answer