10 thoughts on “D79 – Pierce

    1. Receiving a “hit” in our lab meant that our data was out of two standard deviations (could be either positive or negative). It showed that the compound we were testing (terpanin-4-ol) was against the biological target and resulted in killing our bacteria.

    1. We would have to test for the therapeutic index by testing the therapeutic effect (otherwise known as ED50) and the toxic effect (TD50) in order to determine the therapeutic index itself. We know from research that Tea Tree Oil is toxic in its pure form if taken orally, however we did an extraction of the compound Terpinen-4-ol and are unaware if the compound itself if toxic or not. In further testing there could be research to determine the toxicity level of the compound itself.

    1. The incubation period for this lab was a constant variable throughout most of the people in our lab. When we performed the bactericidal/bacteriostatic trial, we chose to do a 48 hour incubation period to determine if more bacteria had died when incubated in a longer period of time. Our results had shown that when we had incubated our compound for a longer time period, slightly more bacteria had died.

    1. We could maximize the efficiency of Terpinen-4-ol by performing an extraction. We performed an ethanol extraction with different dilutions beginning at 70% and 50% ethanol and found that it killed our compound completely, so we performed all of our testing with a 30% ethanol dilution with our compound, which successfully extracted our compound without killing it. This had efficiently shown that the compound terpinen-4-ol had killed the salmonella bacteria during our testing.

  1. Great job with your presentation! Do you think the way that Terpinen-4-ol is delivered would make a difference in how effective it is? For example, is it better to deliver it orally, intravenously, or by some other method?

    1. Since Terpinen-4-ol is an extraction of Tea Tree Oil, and tea tree oil is naturally toxic if taken orally, we would have to test the toxicity of our extracted compound to see if it is toxic as well or if another component in tea tree oil is what makes it toxic when in its natural state. Further studies would help us reveal how the compound would be able to be taken, and if it is toxic or not. We could perform a TD50 and ED50 test in order to find if it has a high therapeutic index to test the toxicity of the compound itself.

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