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12 thoughts on “D85 – Mullen”
In order to identify inhibitors of intracellular S. Tm replication, wouldn’t you want to conduct parallel chemical screens against S. Tm growing in macrophage-mimicking media and within macrophages rather than max dose and dose-response curve experiments?
We were testing not only the effectiveness of drugs killing S. Tm, but also if they were able to be consumed by humans as an antibiotic. That is why the Max Dose experiment was crucial because it allows us to figure out the concentration at which a human can consume the drug before it becomes toxic.
The layout and flow of your poster is really nice. Do you think you could’ve cut out some wording on the abstract and methods sections? I think doing so and making your images larger with the extra space would be a good idea for the future.
Thank you! We wanted to make sure our methods were as clear as possible in case someone were to try our experiment for themselves. However, we could have removed excess information in order to enlarge the pictures and give descriptions for them. I will remember this for the future!
Do you have any hypothesis as to why Azacitidine and drugs of similar structure were not effective in killing Salmonella?
What would you say your biggest takeaway is from this experiment and how might you use the knowledge yo gained in the future?
Do you have any hypothesis as to why Azacitidine and other drugs with similar structures are ineffective in killing salmonella?
Considering the antimicrobial properties, we hypothesized the drug would work on other types of bacteria but our data concludes that it does not kill salmonella. If we had tested at higher concentrations it may have worked but we wanted to make sure the drug was safe to be consumed by humans so if the concentration was too high it would become toxic.
What would you say your biggest takeaway was from this experiment, and how will you use the knowledge you gained in the future?
My biggest take away from this experiment is to make sure your procedure and how you’re doing the experiment is as accurate as possible. A lot of our data was invalid because there was human error and the bacteria did not grow so in the future I would do each experiment as carefully as possible and make sure I write down what I am doing in order to repeat the experiment if need be.
What do you think, chemically or physiologically, could have been the reason your compound didn’t work?
I believe our compound wasn’t successful because we tested it at such a low concentration. We did this to ensure that it would be safe for humans to consume but it could be why it was not successful.