Since your compound was a hit at the physiological relevant “max” dose, do you think this could be a problem when introduced to human cells of being too high of a concentration? Great job 🙂
Hey Nolan! Great question. While the compound itself is inherently toxic in its most pure form, we are fairly certain that even in a solution with a high concentration of Mitomycin it will pose very little threat to a human host. However, this is a testable question. Testing for the toxic and effective doses will allow us to find the therapeutic index that is the most effective and safe dosage. I do not believe that in a solution that even at its highest concentration Mitomycin will pose a threat to a human host.
Hey Sami, to answer both you questions this level of toxicity would be measured in a therapeutic index curve. This cure shows an effective and toxic dose. Where they meet is the level that is ideal for antibiotic usage. However, anything above this point, we would deem too toxic for usage. If these curves do not align then the drug is not capable of being used as an antibiotic. We need to test for this before we can determine what level is too toxic for human usage. This test is typically done through lab rats and mice infected with Salmonella. Hope that answers your questions!
Hey Kaitlyn, while this is beyond the scope of our own research I have a possible method for investigating this. Because of gram straining processes we can locate Salmonella in a solution. After introducing Mitomycin to a solution containing the bacteria and a full incubation period, we can stain the bacteria and locate it in a solution. DNA can be seen in bacteria cells through a microscope. If we were to compare an image of healthy Salmonella and Mitomycin infected Salmonella, we could see the difference in the amount of DNA. If there is more DNA present we can determine that Mitomycin is preventing separation, however if an excess is not seen, we can assume the toxicity of Mitomycin is what is killing the bacteria.
Since your compound was a hit at the physiological relevant “max” dose, do you think this could be a problem when introduced to human cells of being too high of a concentration? Great job 🙂
Hey Nolan! Great question. While the compound itself is inherently toxic in its most pure form, we are fairly certain that even in a solution with a high concentration of Mitomycin it will pose very little threat to a human host. However, this is a testable question. Testing for the toxic and effective doses will allow us to find the therapeutic index that is the most effective and safe dosage. I do not believe that in a solution that even at its highest concentration Mitomycin will pose a threat to a human host.
What is the threshold of toxicity that would deem your treatment too toxic to use in humans?
How toxic would your treatment have to be in order for it to be non usable in humans? What threshold/limits are you setting
Hey Sami, to answer both you questions this level of toxicity would be measured in a therapeutic index curve. This cure shows an effective and toxic dose. Where they meet is the level that is ideal for antibiotic usage. However, anything above this point, we would deem too toxic for usage. If these curves do not align then the drug is not capable of being used as an antibiotic. We need to test for this before we can determine what level is too toxic for human usage. This test is typically done through lab rats and mice infected with Salmonella. Hope that answers your questions!
How would you begin your research on how Mitomycin affects the DNA replication process of Salmonella?
Hey Kaitlyn, while this is beyond the scope of our own research I have a possible method for investigating this. Because of gram straining processes we can locate Salmonella in a solution. After introducing Mitomycin to a solution containing the bacteria and a full incubation period, we can stain the bacteria and locate it in a solution. DNA can be seen in bacteria cells through a microscope. If we were to compare an image of healthy Salmonella and Mitomycin infected Salmonella, we could see the difference in the amount of DNA. If there is more DNA present we can determine that Mitomycin is preventing separation, however if an excess is not seen, we can assume the toxicity of Mitomycin is what is killing the bacteria.
what were some of the limitations to this study? Were results reproduced?