Why do you think your max dosage experiment had a higher percent survival than your serial dilution experiment? If this was not due to experimental error, would that indicate that a lower dosage of your compound would work better as a chemotherapy?
Yes great question!! So there are actually some drugs that have a U shaper dose response, meaning really high effects at really low dosages or really high dosages. Biologically, this opposite response could be due to the compound activating a pathway that leads to survival at high concentrations. At low concentrations it could be activating an opposite pathway that leads to death. So we think that this is a result of a U shaped dosage curve for the compound.
I definitely think that doing more iterations of our Andrographolide max dosage experiment is the most feasible!! This is something we could do that would allow us to better understand how this drug works.
Yes and no. For andrographolide our serial dilution results produced hits that were extremely successful and indicative of a potential chemotherapeutic, however it was shown to have no synergistic effects. So andrographolide could be researched further but probably not for use in combinatorial therapy. Andrographis on the other hand was shown to have a high synergistic effect but very little promise on its own so it could also warrant further investigation but for combinatorial therapy, not as an individual chemotherapeutic.
Great presentation Shreya! What kinds of further experiments would you do with Andrographolide to determine whether or not it has a synergistic relationship with colchicine?
Thank you so much! So I would assume we would have to use the TD50 and ED50 of colchicine and do serial dilutions to determine a suitable concentration. And then we would have to do the same thing for Andrographolide and then the two together. We haven’t really talked too much about it, but it is definitely something we could look into.
So in this experiment, we didn’t take other chemos into account, however the whole goal was also to determine how it DOES interact with other treatments (aka radiation) to see if we can use that interaction as an ADVANTAGE for the treatment of cancer.
Why do you think your max dosage experiment had a higher percent survival than your serial dilution experiment? If this was not due to experimental error, would that indicate that a lower dosage of your compound would work better as a chemotherapy?
Yes great question!! So there are actually some drugs that have a U shaper dose response, meaning really high effects at really low dosages or really high dosages. Biologically, this opposite response could be due to the compound activating a pathway that leads to survival at high concentrations. At low concentrations it could be activating an opposite pathway that leads to death. So we think that this is a result of a U shaped dosage curve for the compound.
Which of your future directions do you think is most feasible in the short-term?
I definitely think that doing more iterations of our Andrographolide max dosage experiment is the most feasible!! This is something we could do that would allow us to better understand how this drug works.
So what are the implications of your findings? Does this synergism make your drug a solid candidate for use in chemo?
Yes and no. For andrographolide our serial dilution results produced hits that were extremely successful and indicative of a potential chemotherapeutic, however it was shown to have no synergistic effects. So andrographolide could be researched further but probably not for use in combinatorial therapy. Andrographis on the other hand was shown to have a high synergistic effect but very little promise on its own so it could also warrant further investigation but for combinatorial therapy, not as an individual chemotherapeutic.
Great presentation Shreya! What kinds of further experiments would you do with Andrographolide to determine whether or not it has a synergistic relationship with colchicine?
Thank you so much! So I would assume we would have to use the TD50 and ED50 of colchicine and do serial dilutions to determine a suitable concentration. And then we would have to do the same thing for Andrographolide and then the two together. We haven’t really talked too much about it, but it is definitely something we could look into.
Sometimes treatments that occur concurrently interact badly. Did you, or how will you take this into account?
So in this experiment, we didn’t take other chemos into account, however the whole goal was also to determine how it DOES interact with other treatments (aka radiation) to see if we can use that interaction as an ADVANTAGE for the treatment of cancer.
referencing your previous reply, how do you think conducting this experiment on those pre exposed to chemo would change the outcome?