There was a good in depth explanation. My question is just to clarify the final results, which got a little muddy among the detailed interpretation of results. Did the outcome contradict your hypothesis? Meaning, did the Curcumin actually enhance bacterial growth?
The outcome contradicted my hypothesis. The hypothesis was that Curcumin would inhibit the growth of the bacteria; however, the bacteria grew at a high absorbance. Although further testing is needed, a statistical hit showed that the 10mg/mL concentration of Curcumin lead to abnormal bacterial growth and suggests that it was enhancing bacterial growth.
Do you think that using this as an antibiotic at a certain dosage can prove fatal to people? Do you have any idea if the antibiotic effects of curcumin vary based on dosage? Does a high dose always work better?
While further testing is needed, if Curcumin does promote bacterial growth (as the statistical hit for the 10mg/mL concentration suggested), it could potentially be dangerous to use during an infection. It seemed that no antibiotic effects were present at any dosage tested for Curcumin in our experiment. A high dose does not always work better, as a drug can grow lethal if is used in a higher concentration. Each drug has a therapeutic index that shows the relationship between the therapeutic and toxic effects of the drug. Because of this relationship, drugs can grow fatal as the dosage increases.
How does the yellow color effect absorption exactly? In the figure with yellow bars, is the concentration of your compound increasing or decreasing left to right?
While further testing is needed to show specifically how the yellow coloring affects absorption values, since the absorption is read using spectrophotometry at a wavelength of 620nm and the color yellow is very close to this wavelength, at 580nm, it is possible that it raised the absorbances detected from each Curcumin concentration (and thus the amount of ‘presumed’ bacteria present). The concentration is decreasing from left to right, from 10 to 8,4,2, and 1mg/mL.
There was a good in depth explanation. My question is just to clarify the final results, which got a little muddy among the detailed interpretation of results. Did the outcome contradict your hypothesis? Meaning, did the Curcumin actually enhance bacterial growth?
The outcome contradicted my hypothesis. The hypothesis was that Curcumin would inhibit the growth of the bacteria; however, the bacteria grew at a high absorbance. Although further testing is needed, a statistical hit showed that the 10mg/mL concentration of Curcumin lead to abnormal bacterial growth and suggests that it was enhancing bacterial growth.
Do you think that using this as an antibiotic at a certain dosage can prove fatal to people? Do you have any idea if the antibiotic effects of curcumin vary based on dosage? Does a high dose always work better?
While further testing is needed, if Curcumin does promote bacterial growth (as the statistical hit for the 10mg/mL concentration suggested), it could potentially be dangerous to use during an infection. It seemed that no antibiotic effects were present at any dosage tested for Curcumin in our experiment. A high dose does not always work better, as a drug can grow lethal if is used in a higher concentration. Each drug has a therapeutic index that shows the relationship between the therapeutic and toxic effects of the drug. Because of this relationship, drugs can grow fatal as the dosage increases.
How does the yellow color effect absorption exactly? In the figure with yellow bars, is the concentration of your compound increasing or decreasing left to right?
While further testing is needed to show specifically how the yellow coloring affects absorption values, since the absorption is read using spectrophotometry at a wavelength of 620nm and the color yellow is very close to this wavelength, at 580nm, it is possible that it raised the absorbances detected from each Curcumin concentration (and thus the amount of ‘presumed’ bacteria present). The concentration is decreasing from left to right, from 10 to 8,4,2, and 1mg/mL.