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16 thoughts on “G1 – Arnold”
What defines a slippery sequence?
A slippery sequence is normally about 9 nucleotides that is generally rich in A and G. There are several variations, but they are what causes the frameshift
What is it about glycine and lysine that allows for slippage?
good job on your presentation, i have just one question, what is a chaperone protein? you mentioned it in your conclusion that you found that every single L cluster had -1 frameshift that completely shifts the relatively “chaperone protein”.
Thank you, I was referring to the tail assembly chaperone which aids in the functional assembly of progeny in the late lytic cycle.
Why do all L1 cluster phages have an -1 frameshift in the 2 tail assembly?
Based on the abundance of the shift, it is integral to the phages ability to bind and infect the bacterial host.
How would you go about completing your next stage of research, whether that be comparing it to other bacteriophage operons or investigating other forms of slippery sequence?
We would like to use the same bioinformatics programs used, just on a larger scale to investigate the frequency in other clusters and which slippery sequence causes it where.
Really great and interesting presentation! What do you think causes a slippery sequence like this?
A slippery sequence is encoded in the DNA intentionally as part of the genome to cause the functional gene to be translated
Could this frameshift occur in phages other than L cluster phages, or is it only found largely in L cluster. What evolutionary factors could have resulted in this?
We only investigated the L cluster, but it can occur in any phage. It likely occurs in many more phage that we did not investigate. Through the process of horizontal integration and evolution, there are a large range of factors that could cause the slippery sequence to become part of the phage’s genomic sequence.
Why does this frameshift occur in all L cluster phages?
Because it is vital to its ability to infect and reproduce.
Hi, great presentation? What made you decide to look at L1 tail assemply chaperones?