8 thoughts on “G13 – O’Sullivan

  1. I was wondering, how exactly you would plan to go about determining which specific metal ion is responsible for binding?

    1. Hi! I actually did some research and there is a metal-binding site validation tool called CheckMyMetal (CMM) that analyzes any metal binding site in a macromolecule and can tell you the metal ion that is bound. It can also show any irregularities or incorrect metal assignments which is pretty cool! So, I would probably run the metallophosphoesterase through this program to see which metal ion is bound.

  2. Nice job! This sounds like very important work. Could you describe how these findings could be applied in terms of how the phages might be used based on degree of conservation?

    1. Hi! Thank you! I think that confidently determining conservation of these proteins will allow us to prove they share a similar function and therefore get a better idea of their role in a phage genome. The better we understand these phages, the more confidently we can annotate them and hopefully apply them to phage therapy (to combat antibiotic-resistant illnesses).

  3. Great presentation! What specific research could you do to perform your future direction of identifying which which specific cellular process this region
    is responsible for?

    1. Hi! I would run it through a program (called CheckMyMetal) to determine the metal ion that is bound to this specific metallophosphoesterase. From there, I can narrow down the process that the Helix-turn-Helix DNA Binding Domain Protein and metallophosphoesterase work together in because different metal ions contribute to different processes. I couldn’t tell you off the top of my head which metal ions contribute to which process, however, further research would definitely allow me to do so.

  4. Great presentation and poster! Can you comment on specific substrates that this metallophosphoesterase might bind? How does it compare to nucleases and phosphatases?

    1. Hi! Thank you for listening! The metallophosphoesterase could bind ions like manganese, magnesium, iron, cobalt, zinc, nickel, etc. The bound ion is essentially determines which process this metallophosphoesterase could play a role in. Honestly, I would say it functions very similarly to a phosphatase (due to their interactions with phosphate groups), there are even certain types of phosphatases that are thought to have evolved from the metallophosphoesterase superfamily. As far as nucleases go, I would say they are less similar because nucleases specifically deal with nucleic acids whereas metallophosphoesterases interact with phosphate groups in order to achieve their goal.

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