8 thoughts on “G4 – Felfli

    1. Hi McKenna! Since there are two open reading frames in the TAC gene, when the ribosome goes to translate the sequence and produce a protein, a different sequence is actually read by the ribosome, which results in two different proteins. We can also use bioinformatic tools like NCBI-BLAST and HHPred to identify the gene products as proteins produced by the TAC gene. Hopefully that answered your questions!

  1. Is having two open reading frames on this gene simply a means for the phage to save space within its genome?

    1. Hi Calvin! Having overlapping genes does allow the phage to save space within its genome to a certain extent since there is a limited amount of space for the bacteriophage’s genome within a fully formed capsid, however, with the TAC gene, the two open reading frames actually help to also control the proportion of how much of each protein is made: the protein made by the first ORF is produced less than that of the second ORF and controlling the ratio of these proteins helps to assure that the phage tail is formed correctly.

  2. How would you go about artificially producing the tail assembly chaperone protein in vivo?

    1. Hi Jackson! That’s a good question. I can’t say I know exactly what that process would look like, but I think perhaps taking the genome of a phage and making a mutation that would “reflect” the gene sequence we identified in our research rather than making an entire genome from scratch would be a simpler way of going about this. I’ll need to do more research!

    1. Hi Brandt! As of this moment, I don’t believe there is a lot of research surrounding how the size of overlap would affect the proteins produced, however some believe that gene overlap occurs to “save space” so to speak in the genomes of the bacteriophages, since the genome can only be so large.

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