Hi Laura, we hope that investigating more genes in the L1 subcluster would provide with further information about the regions that Enceladus is missing. Because Enceladus seems to be much shorter in total genome length compared to other L1 subcluster phages, the genes that Enceladus does not have may give further information as to where the divergence between the phages of that cluster had happened.
Hi Adriona, We did not really encounter any problems when annotating Enceladus. But it was initially very challenging when starting to learn how to use all the bioinformatics tools and interpret the results we were being given.
Hi Molly, I think it would be interesting to look at the functions, and any potential functions that they may have within bacteriophages. The Whib transcriptional factors have a clear function in bacteria, but not so much in bacteriophages. So its interesting to look at possible inheritance and really see if there is a function at all. Does it have a function or was it merely transferred from the bacteria to the phage?
Hi Savannah, I do not know the exact efficacy rate. However, I think if most of the variables are controlled, it would give at least some indication as to the function of these genes. Doing this with a large data set (lots of bacterial lawns and banctiophages) would help mitigate any variation due to random uncontrollable variables.
Why is it more beneficial to investigate more genes in the L1 subcluster?
Hi Laura, we hope that investigating more genes in the L1 subcluster would provide with further information about the regions that Enceladus is missing. Because Enceladus seems to be much shorter in total genome length compared to other L1 subcluster phages, the genes that Enceladus does not have may give further information as to where the divergence between the phages of that cluster had happened.
Great presentation! What problems did you encounter, if any, and how did you solve them?
Hi Adriona, We did not really encounter any problems when annotating Enceladus. But it was initially very challenging when starting to learn how to use all the bioinformatics tools and interpret the results we were being given.
What results would you ideally hope to see from the future research on genes 75 and 76?
Hi Molly, I think it would be interesting to look at the functions, and any potential functions that they may have within bacteriophages. The Whib transcriptional factors have a clear function in bacteria, but not so much in bacteriophages. So its interesting to look at possible inheritance and really see if there is a function at all. Does it have a function or was it merely transferred from the bacteria to the phage?
Well done! Do you know the efficacy rate of a gene knockout? Does it effectively determine the exact function within each phage?
Hi Savannah, I do not know the exact efficacy rate. However, I think if most of the variables are controlled, it would give at least some indication as to the function of these genes. Doing this with a large data set (lots of bacterial lawns and banctiophages) would help mitigate any variation due to random uncontrollable variables.