7 thoughts on “P6 – Wood

    1. We got it from the banks of a lake in Niwot. M. smegmatis is often found in freshwater and soil environments so we looked for a combination of the two.

    1. From our existing isolated high titer lysate, we would create a new DNA isolate sample. We would redo our initial PCR procedure from there by combining the DNA with sub cluster A4 primers and letting the sample undergo cycles of denaturing, annealing, and extending. We would redo our negative control by just running the primers on the gel and redo our positive control by combining known cluster A4 DNA with the primers, running that alongside the other three samples.

  1. What kind of results would you expect to see if the experiment was redone at around room temp (20-25 degrees Celsius)?

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