We got it from the banks of a lake in Niwot. M. smegmatis is often found in freshwater and soil environments so we looked for a combination of the two.
From our existing isolated high titer lysate, we would create a new DNA isolate sample. We would redo our initial PCR procedure from there by combining the DNA with sub cluster A4 primers and letting the sample undergo cycles of denaturing, annealing, and extending. We would redo our negative control by just running the primers on the gel and redo our positive control by combining known cluster A4 DNA with the primers, running that alongside the other three samples.
Where did you get your soil sample from and why there?
We got it from the banks of a lake in Niwot. M. smegmatis is often found in freshwater and soil environments so we looked for a combination of the two.
Was there a reason for naming the phage KingJellyfish?
We originally wanted to name it just “Jellyfish” but that was taken
How would you redo your PCR to have it show conclusive results about the A4 cluster?
From our existing isolated high titer lysate, we would create a new DNA isolate sample. We would redo our initial PCR procedure from there by combining the DNA with sub cluster A4 primers and letting the sample undergo cycles of denaturing, annealing, and extending. We would redo our negative control by just running the primers on the gel and redo our positive control by combining known cluster A4 DNA with the primers, running that alongside the other three samples.
What kind of results would you expect to see if the experiment was redone at around room temp (20-25 degrees Celsius)?