Great job! What exactly would running a quality control gel help you determine/clear up? Would it be useful information to the research you presented, or would it open up new avenues of research into your phage?
Hi there Tyler! Running a quality control gel would allow us to isolate the DNA of our phage. We would then use the isolated DNA to run a PCR. Through the PCR, we will be able to identify the cluster of our phage.
Hey Alli! First, I really liked the name of your phage its fun and catchy to say! Second, I was wondering if there was anything else other than the long flexible tail that indicated that it was apart of the siphoviriade?
Thank you! The name GoofyGoober is actually a reference to SpongeBob. The structure of the bacteriophage determines its classification. Siphoviridae phages have heads that are around 60nm in diameter. Our phage had a head that was 64.18 nm long.
Running a quality control gel would allow us to isolate the DNA of our phage. We would then use the isolated DNA to run a PCR. Through the PCR, we will be able to identify the cluster of our phage. Knowing this information would allow people accessing the database to find our phage as a candidate.
Great job! What exactly would running a quality control gel help you determine/clear up? Would it be useful information to the research you presented, or would it open up new avenues of research into your phage?
Hi there Tyler! Running a quality control gel would allow us to isolate the DNA of our phage. We would then use the isolated DNA to run a PCR. Through the PCR, we will be able to identify the cluster of our phage.
Hi Alli! I’m curious if you were able to get a plaque to show up on your plate on the first try, or if it took multiple attempts?
Hey there Anza! It took 2 attempts to get a plaques to show up on our plate.
Hey Alli! First, I really liked the name of your phage its fun and catchy to say! Second, I was wondering if there was anything else other than the long flexible tail that indicated that it was apart of the siphoviriade?
Thank you! The name GoofyGoober is actually a reference to SpongeBob. The structure of the bacteriophage determines its classification. Siphoviridae phages have heads that are around 60nm in diameter. Our phage had a head that was 64.18 nm long.
Great Presentation! My biggest question is what information would you had gotten if you had ran a quality control?
Running a quality control gel would allow us to isolate the DNA of our phage. We would then use the isolated DNA to run a PCR. Through the PCR, we will be able to identify the cluster of our phage. Knowing this information would allow people accessing the database to find our phage as a candidate.
Hi Alli! Had you phage been lysogenic rather than lytic, how would that influence your future experiment?