8 thoughts on “P62 – Ribeiro

  1. Great presentation overall, though I am somewhat curious about why the different life cycles of the phage matter.

    1. The lifecycles are important because the lytic bacteriophage is the one that kills the cell. If our isolated bacteriophage was temperate, it would undergo the lysogenic lifecycle, not killing the cell. So, if we used a temperate phage for phage therapy it would not be effective since it would integrate it’s genome into the genome of the host cell, not killing it.

  2. What were your expected outcomes? How were those different from the results you found in your experiment?

    1. Our expected outcomes were the same as the results we found in our experiment. We expected that our phage was going to be lytic or temperate and it was lytic. Since it was lytic, we expected that it would lyse the cell, as it did.

  3. Great job! What are some further experiments you could perform to test the effectiveness of your phage’s ability to infect hosts?

    1. All the experiments that could test the effectiveness of our phage were already done on our dilution, spot test, web pattern plate.

    1. Mycobacterium Smegmatis is a good host to study because it has a wide host range and it is also non-pathogenic and from the same family as M. tuberculosis, which means it is safer to study at the laboratory.

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