8 thoughts on “P64 – Huetson

  1. Hi Delaney,
    What kind of disturbance might cause a phage in the lysogenic cycle to transition into the lytic cycle?

    1. Hi Eva,
      Thanks for the question. One way to induce the temperate phage to transition to lytic lifecycle is to make environmental stressors such as raising temperatures, or to genetically modify the phage and remove the repressor gene that represses the lytic lifecycle.

  2. If you could continue this experiment, where do you think you would look next? Would you choose this phage again?

    1. If we continued this experiment we would most likely isolate the DNA and then perform a restriction digest in order to narrow down the cluster of our phage. I would choose to do more experimentation with temperate phage in order to further research their use in the medical field and be more utilized in phage therapy.

    1. Hi Madison,
      Thank you for the question. We determined the family based EM imaging that showed an isometric head of the phage and relatively long tail for phages. Both features are characteristics of the Siphoviridae family.

  3. Hey Delaney,

    Great presentation! You did a great job thoroughly explaining all of the information. One question I did have, are there specific factors that can cause the lysogenic –> lytic conversion? Can there be multiple factors that induce the conversion, or is it typically due to a single stressor?

    1. Hi Taylor,
      Thank you for the great questions. There are multiple ways to induce the lytic lifecycle in these temperate phage. We can make environmental stressors like raising temperatures and hope to induce to lytic lifecycle, but the more used way is to delete the repressor gene that stops the lytic lifecycle from occurring.

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