There is not necessarily an ideal phage tail size. Each phage tail size is just unique to a different phage or within a range for a specific phage type. Mine is just long in comparison to others, tail sizes have been small as 89nm for this type, and mine was just on the longer side.
This phage personally was found at a higher temperature, so I was just curious as to if it would be more or less effective in a different environment than the one it was found it.
I know some environmental factors can cause phage to go from lysogenic to lytic cycle, however my phage is already lytic. But i’m assuming if temperature has any other effect on phage infection, building, or any other component, its sucess in fighting disease through such mechanisms would also be altered.
What do you think lead to the contamination in your restriction digest experiment? Also, what would the experiment of testing in colder temperature possibly lead you to in terms of extra conclusions or hypothesis’?
A number of things could have caused contamination; unsterile environment or tips, misuse of components, etc. I personally think, however, that it was a cross contamination of a community material like cutsmart, since others in class experienced similar things. And if I were to find phage success was dependent on temperature, This could possibly contribute to uses against bacteria by using phage in optimal temperatures.
You mentioned the tail of your phage was long. What’s the ideal tail size?
There is not necessarily an ideal phage tail size. Each phage tail size is just unique to a different phage or within a range for a specific phage type. Mine is just long in comparison to others, tail sizes have been small as 89nm for this type, and mine was just on the longer side.
Is there any specific reason you want to test your phage’s infection rates at lower temperatures, or is it just out of curiosity?
This phage personally was found at a higher temperature, so I was just curious as to if it would be more or less effective in a different environment than the one it was found it.
Is there a significance to the importance of temperature to a phage in terms of their effects on specific diseases?
I know some environmental factors can cause phage to go from lysogenic to lytic cycle, however my phage is already lytic. But i’m assuming if temperature has any other effect on phage infection, building, or any other component, its sucess in fighting disease through such mechanisms would also be altered.
What do you think lead to the contamination in your restriction digest experiment? Also, what would the experiment of testing in colder temperature possibly lead you to in terms of extra conclusions or hypothesis’?
A number of things could have caused contamination; unsterile environment or tips, misuse of components, etc. I personally think, however, that it was a cross contamination of a community material like cutsmart, since others in class experienced similar things. And if I were to find phage success was dependent on temperature, This could possibly contribute to uses against bacteria by using phage in optimal temperatures.