View the poster here. Share this:Click to share on Twitter (Opens in new window)Click to share on LinkedIn (Opens in new window)Like this:Like Loading...
11 thoughts on “P8 – Dollarhide”
Hi! How many families of Phages are there?
I’m not exactly sure how many families of phage exist in the scientific world, but I know that the way we can narrow down which family our phage is in is by observing the physical qualities of our phage with electron microscopy.
what led you to concluding that you were not successful in getting your own phage?
My partner and I knew that we were not successful in obtaining our own phage when all of our enrichment experiments resulted in no plaques.
Could you go into a little more detail as to how the serial dilution allows you to isolate a single morphology of plaques?
Hi! Our serial dilutions allow us to isolate a single morphology of plaques because as we dilute our solution, we are able to decrease the amount of phage DNA in each dilution, resulting in a very dilute and isolated phage sample.
Could you explain what is a “DNA cutting pattern”?
A DNA cutting pattern is when we run a restriction digest with different enzymes, and these specific enzymes cut our phage DNA into different segments.
What made your restriction digest experiment not work? was it that the cluster that matched the BAMH1 cuts did not match any of the others?
Hi! I cannot be completely sure as to why our restriction digest experiment did not work, but one possibility is that we had some trouble with our gel which we used in the experiment and that may have resulted in the lack of DNA cuts. The only way to be sure what cluster our phage belongs to would be to use another gel and repeat the experiment.
I’m not sure if my other reply came through, but just in case it did not, our restriction digest experiment did not succeed because we used a faulty gel which resulted in a lack of DNA cuts. The only way to know for sure which cluster our phage belongs to would be to repeat the experiment.